Tracking the information about your manuscript
Communicate with the editorial office
Query manuscript payment status Editor LoginCollecting, editing, reviewing and other affairs offices
Managing manuscripts
Managing author information and external review Expert Information Reviewer LoginOnline Review
Online Communication with the Editorial Department
Developments
Editorial Department of "Chemistry ＆ Bioengineering" has been Awarded "Advanced Collective for Studying and Implementing the Spirit of the Third Plenary Session of the 20th Central Committee of the Communist Party of China" by Hubei Provincial Editology Society for Science Periodicals in 2024
A Paper from "Chemistry ＆ Bioengineering" Selected as One of Hubei Province's Top 100 Outstanding Papers
The 8th "Hubei Young Scientists Forum in Chemistry and Chemical Engineering" Successfully Held in Xianning
more..
Research Progress on Adhesive Hydrogels in Tissue Defect Repair
SHU Hangqi;YU Mengqiu;HE Yulong;WANG Yiyu;ZHAO Kai;The key to repairing tissue defects lies in timely closure of the wound and hemostasis.Traditional clinical approaches including surgical suture, staple suture, etc. are associated with limitations such as the secondary surgical removal, inadequate sealing, susceptibility to scarring, low adhesive strength, and suboptimal biocompatibility.Adhesive hydrogels are three-dimensional polymer network materials, which have developed rapidly in recent years.Due to strong adhesion, excellent biocompatibility, and degradability, adhesive hydrogels have solved the problems existing in traditional clinical approches and promoted the development and application in the field of tissue defect repair.We elaborate the mechanism of adhesion between hydrogel and tissue, including physical, chemical, and mussel-inspired effects.Then, we summarize the research progress of adhesive hydrogels in the defect repair of various tissues such as skin, periodontal, bone, heart, and cornea in recent years.Finally, we analyze the challenges faced by adhesive hydrogels, and put forward prospects for the future development directions.
Research Progress in Bacterial Degradation of Polyester Plastics and Its Hydrolase
YOU Lelan;HUANG Xu;CHEN Xiaochen;YANG Xiaoru;WANG Lei;WANG Xinhong;SU Jianqiang;The use of plastic products in industry and daily life has far exceeded that of materials such as wood and steel.With the global understanding of the environmental risks of plastics and the promotion of plastic restriction programs, the use of polyester plastics in production and life has gradually increased because it contains ester bonds that can be hydrolyzed by microorganisms.The microbiological degradation of polyester plastics and the engineering modification and performance improvement of polyester hydrolase are research hotspots in this field.We review the research progress in bacterial degradation of traditional and biodegradable polyesters, analyze and summarize the biodegradability of polyesters, the taxonomic distribution of polyester-degrading bacteria, and the engineering modifications of algorithm-assisted polyester hydrolase, which provides the references for pollution control and recycling of environmental polyester plastics.
Synthesis of High-Entropy Alloy Catalyst(Ni_3MoCoZn)Al12O24 by Sol-Gel Method and Its Application in Dry Reforming of Methane
XU Xin;BAI Yue;LI Lin;To address the issues of carbon deposition and activity decay in dry reforming of methane(DRM),we characterized the structure of high-entropy alloy Ni-based catalysts(Ni_3MoCoZn)Al12O24 synthesized by hot solvent method, co-precipitation method, ball milling method, and sol-gel method, and compared their DRM reaction catalytic performances.The results show that(Ni_3MoCoZn)Al12O24 catalyst synthesized by sol-gel method has a larger specific surface area(about 118 m2·g-1) and a favorable mesoporous structure(3-4 nm).The catalyst synthesized by sol-gel method has a higher proportion of high-temperature reduction peaks, suggesting it has a stronger metal-support interaction.The catalyst synthesized by sol-gel method exhibits the highest and the most stable conversion rates of CH4 and CO2 at 800 ℃ and GHSV of 36 000 mL·h-1·gcat-1.While, the catalysts synthesized by co-precipitation method and ball milling method show significant deactivation after 20 h.This study provides a experimental evidence for the design, synthesis, and the element regulation of DRM catalyst.
Construction of A Heterogeneous Electro-Fenton System Using FeMn-MOFs@GF Cathode for Degradation of Enrofloxacin
DENG Qiang;LIU Shanshan;WANG Xiaoyuan;LIU Jintian;HOU Huangli;DAI Jie;TIAN Zhihong;To efficiently treat antibiotic wastewater, we prepared Fe_xMn7-x-MOFs@GF cathode through modification of graphite felt(GF),established a heterogeneous electro-Fenton system, and systematically investigated its degradation performance and mechanism for Enrofloxacin(ENR).The results show that, when the calcination temperature is 600 ℃ and the molar ratio of Fe to Mn is 4∶3,the degradation performance of Fe_4Mn3-MOFs@GF cathode for ENR is the best.Under the conditions of the initial pH value of 5.0, the current density of 15.24 mA·cm-2,and the aeration rate of 2.0 L·min-1,the removal rate of ENR reaches 97.89% at 120 min.After 6 cycles, the removal rate of ENR by Fe_4Mn3-MOFs@GF cathode only decreases by 7.16%,achieving the stable degradation of ENR.
Secondary Metabolites from Co-Culture of Two Algicolous Trichoderma Strains
ZHANG Wei;SHI Zhenzhen;JI Naiyun;We co-cultured two algicolous Trichoderma strains(Trichoderma virens and Trichoderma longibrachiatum) isolated from Zhoushan Sea area, and obtained eight compounds through separation and purification of their crude fermentation extracts by using reverse-phase silica gel column chromatography, silica gel column chromatography, and gel column chromatography.Based on ~1HNMR,13CNMR analysis, and comparison with literature data, these compounds are identified as CAF-603(1),trichocarane A(2),trichocarotin H(3),trichocarotin N(4),7-β-hydroxy CAF-603(5),trichocarotin Ⅰ(6),(3R,5R)-3,5-dihydroxydecanoic acid(7),and 3,5-dihydroxydecanoic acid methyl ester(8).
Construction of An Indium-Modified Weakly Alkaline Aqueous Iron-Chromium Redox Flow Battery and Its Energy Storage Performance
LIU Yingying;ZHANG Xiaofei;LUO Jingyao;ZHU Zonghan;FENG Qinghao;LIU Xueyi;LI Yafei;XIE Xiaoyin;DENG Xiangyi;We prepared a CrPDTA chelate by the chelation reaction of PDTA with chromium ion,obtained an indium-modified graphite felt electrode by modifying graphite felt electrode with indium chloride, and characterized the CrPDTA chelate and indium-modified graphite felt electrode by FTIR,UV-Vis,SEM,and EDS.Moreover,we assembled an indium-modified weakly alkaline aqueous iron-chromium redox flow battery(InICRFB) by using indium-modified graphite felt electrode,K4 Fe(CN)6 as a positive electrolyte and CrPDTA as a negative electrolyte,and systematically investigated the effects of indium-modified graphite felt electrode on the key performance indicators of the battery,such as energy efficiency,voltage efficiency,and capacity retention rate,etc.Furthermore,we preliminarily explored the internal mechanism of the performance enhancement.The results show that there is a chelation between PDTA and chromium ions,and indium is uniformly attached to the surface of graphite felt electrode.Indium plays a key role in improving the electrode conductivity and reducing charge transfer losses.Compared with the pristine ICRFB,In-ICRFB has the better battery performance:after 200 charge-discharge cycles at a current density of 50 mA·cm-2,the Coulomb efficiency is about 99.0%,the energy efficiency is 81.4%,the voltage efficiency is 82.2 %,the capacity retention rate is 35.0 %,and the average capacity degradation rate of single cycle is 0.325%.
[Downloads: 9 ] [Citations: 0 ] [Reads: 0 ] HTML PDF Cite this article
Anti-Breast Cancer Active Components in Loquat Leaves and Their Inhibitory Effects on Topoisomerase Ⅰ
LIU Jiayao;YANG Junlong;XIE Yuman;HUANG Hui;ZENG Huang;NIE Hua;GUO Bohong;In order to explore the anti-breast cancer active components in loquat leaves and their inhibitory effects on topoisomerase Ⅰ(TOP Ⅰ),we integrated network pharmacology to screen the anti-breast cancer active components from loquat leaves and core pathways.Moreover,we analyzed the interaction mode and inhibitory efficacy of the active components with TOP Ⅰ by using molecular docking and TOP Ⅰ-mediated DNA relaxation experiments.Furthermore,we investigated the effects of the active components on the proliferation,migration,cloning,and apoptosis of human breast cancer cells MCF-7 by cytotoxicity experiments,cell scratch healing experiments,cell cloning experiments,and cell apoptosis experiments.The results show that quercetin,ellagic acid,(-)-epigallocatechin gallate,and kaempferol are the key anti-breast cancer active components in loquat leaves,they can stably bind to the catalytic domain of TOP Ⅰ.All four active components can inhibit the activity of TOP Ⅰ in a concentration-dependent manner.The half-maximal inhibitory concentrations(IC50) of the four active components are 160.70,84.82,72.54,and 80.67 μmol·L-1,respectively,significantly inhibiting the growth,proliferation,migration,and cloning of MCF-7 cells and promoting cell apoptosis.This study provides a theoretical basis for the research of antitumor activity of loquat leaves extracts.
[Downloads: 58 ] [Citations: 0 ] [Reads: 0 ] HTML PDF Cite this article
Research Progress in Carbon-Based Catalysts Modification Strategies and Electrochemical Reactor for Synthesis of H_2O2 via 2e-ORR
LU Zilan;JIA Daqing;HU Huawei;ZHU Leilei;KHAN Asim;XU Wenxuan;LIAO Benren;ZHANG Lehua;The synthesis of hydrogen peroxide(H_2O2) via two-electron oxygen reduction reaction(2e-ORR) has become a new and sustainable alternative to industrial anthraquinone process due to its advantages of normal temperature and pressure operation,green raw materials,and renewable energy.Based on the brief explanation of synthetic mechanism of H_2O2 via 2e-ORR,we review the modification strategies of carbon-based catalysts,including heteroatom doping,defect engineering,functional groups,etc.At the same time,we summarize the structure characteristics and application scenarios of H-type,flow-type,and membrane electrode assembly(MEA) reactors,and point out the challenges faced by the current technology,which provides a theoretical support for the research of H_2O2 synthesis via 2e-ORR.
[Downloads: 77 ] [Citations: 0 ] [Reads: 0 ] HTML PDF Cite this article
Preparation and in vitro Dissolution of Imatinib Liposomes
LIU Chunyu;HU Xuelei;In response to the problems of poor solubility,low oral bioavailability,and adverse reactions caused by Imatinib,we prepared Imatinib liposomes by passive drug-loading and active drug-loading processes,respectively.Moreover,we optimized the process parameters through single-factor experiments by using the encapsulation efficiency as an indicator.Furthermore,we systematically studied the microscopic morphology,particle size,Zeta potential,in vitro release behavior,and stability of Imatinib liposomes.The results show that,in the passive drug-loading process, the encapsulation efficiency of Imatinib liposomes prepared by film dispersion method is the highest[(67.0±2.4)%];in the active drug-loading process,under the conditions of the drugloading time of 40 min,the drug-loading temperature of 50 ℃,the drug-lipid ratio of 1:30,the transmembrane pH gradient of 5,and the ammonium sulfate concentration of 100 mmol·L-1,the encapsulation efficiency of Imatinib liposomes is the highest[(65.0±4.1) %].The liposomes prepared by both two processes have regular morphology,spherical or nearly spherical shapes,with a particle size of 100-110 nm,showing good dispersion.The Imatinib liposomes prepared by the active drug-loading process exhibit good sustained-release effect in vitro and have better stability at 4 ℃ refrigeration than that at room temperature.The study lays a foundation for the development of a new drug delivery system for Imatinib.
[Downloads: 101 ] [Citations: 0 ] [Reads: 0 ] HTML PDF Cite this article
Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[Downloads: 7,597 ] [Citations: 272 ] [Reads: 20 ] HTML PDF Cite this article
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research Progress on Pretreatment Technologies of Lignocellulose
ZHAO Lü1,LI Zhi-guang1,2,LI Hui-yong1,2,XIE Lei1, ZHOU Zhi1(1.College of Science,Hunan Agricultural University,Changsha 410128,China;2.Institute of Cheimstry & Chemical Engineering,Central South University,Changsha 410083,China)This paper reviews the components and structure of lignocellulose,states some promising pretreatment technologies in recent years,evaluates the main pretreatment technologies.
[Downloads: 3,006 ] [Citations: 209 ] [Reads: 14 ] HTML PDF Cite this article
Research Progress of Chlorogenic Acid
DENG Liang, YUAN Hua, YU Zong-yuan(Hubei Research Institute of Chemistry, Wuhan 430074, China)The distribution ,biosynthesis,properties, pharmaceutical activity,the extraction and purifying technology and applied prospect of chlorogenic acid were reviewed in this paper, which tried to provide references for the research of chlorogenic acid, and the production of the medicines comprising chlorogenic acids as well.
Research Advances of Separation and Purification for Protein
WANG Zi-jia1,LI Hong-mei2,GONG Ai-jun1,CAO Yan-qiu1,YUAN Hai-tao1(1.University of Science and Technology Beijing,Beijing 100083,China;2.National Institute of Metrology,Beijing 100013,China)The method of separation and purification for protein is one of the hotspots of the field for life science.This article summarizes many methods of separation and purification,based on the characteristic of protein′s acid-bases,the size and shape of the molecular,the solubility and the compatibility with legends.
[Downloads: 12,053 ] [Citations: 172 ] [Reads: 17 ] HTML PDF Cite this article
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[Downloads: 7,597 ] [Citations: 272 ] [Reads: 20 ] HTML PDF Cite this article
Research Progress of Immobilized Enzyme
CHEN Jian-long~(1,2),QI Jian-cheng~1,CAO Yi-zhi~2,GUO Yong~1,DUAN Hui-li~1(1.Institute of Medical Equipment,Academy of Military Medical Sciences, National Engineering Research Center for Biological Protective Equipment,Tianjin 300161,China;2.School of Life Science,Lanzhou University,Lanzhou 730000,China)The immobilized enzymes have been widely applied in many fields for their more advantages than free enzyme,especially for their stability and reusability.At present,immobilization technologies of enzyme have obtained wonderful development.The research of immobilized enzyme will be a very important development field in the enzyme engineering in the future.In this article,various conventional and new methods for preparation of immobilized enzyme are introduced. At the same time,the immobilization of enzyme on some carriers with excellent properties are reviewed.