Tracking the information about your manuscript
Communicate with the editorial office
Query manuscript payment status Editor LoginCollecting, editing, reviewing and other affairs offices
Managing manuscripts
Managing author information and external review Expert Information Reviewer LoginOnline Review
Online Communication with the Editorial Department
Developments
Editorial Department of "Chemistry ＆ Bioengineering" has been Awarded "Advanced Collective for Studying and Implementing the Spirit of the Third Plenary Session of the 20th Central Committee of the Communist Party of China" by Hubei Provincial Editology Society for Science Periodicals in 2024
A Paper from "Chemistry ＆ Bioengineering" Selected as One of Hubei Province's Top 100 Outstanding Papers
The 8th "Hubei Young Scientists Forum in Chemistry and Chemical Engineering" Successfully Held in Xianning
more..
Research Progress in Pathogenic Mechanisms and Targeted Drugs of Human OX40 and CD30
ZHANG Xilun;SHAO Hongwei;KE Hang;Human OX40 and CD30 belong to the tumor necrosis factor receptor superfamily.We introduce their gene structures, protein domain characteristics, and ligand binding modes, and summarize their expression regulation patterns in immune populations such as T cells and antigen-presenting cells.Moreover, we compare and analyze the dual action mechanisms of OX40 and CD30 in autoimmune diseases and tumors.Furthermore, we systematically review the research and development progresses of targeted drugs for OX40 and CD30:in the field of autoimmune diseases, monoclonal antibodies that antagonize the OX40/OX40L interaction have shown clinical potential in indications such as moderate to severe atopic dermatitis; in the field of tumor treatment, antibody-drug conjugates targeting CD30 have achieved successful applications and promoted the development of new strategies such as bispecific antibodies and cell therapies, while agonist therapies targeting OX40 are dedicated to enhancing anti-tumor immune responses through combination therapy and novel multifunctional antibodies.By integrating basic research and clinical application, this review provides a theoretical basis and research directions for precise immunotherapy targeting inflammation, autoimmune diseases, and tumors.
Monitoring, Early Warning, and Management of Limnoperna fortunei in Water Diversion and Transfer Tunnels
SONG Chiyang;WANG Cong;XIONG Biao;WANG Xidong;CHEN Zhenghu;HUANG Di;XU Tao;HUANG Yingping;LIU Honglin;We systematically review the impacts of Limnoperna fortunei on the tunnel efficiency, maintenance costs, and ecological environments of water diversion and transfer tunnels, and elucidate the potential risks to water conveyance systems.Moreover, we outline the monitoring and early warning techniques based on analysis of water quality parameters and molecular biological detection, aiming to enhance the sensitivity and rapid detection of Limnoperna fortunei invasions, thereby providing critical technical supports for early-stage prevention and control.Furthermore, considering the structure characteristics of water diversion and transfer tunnels, we evaluate the suitable management techniques for different tunnel sections and comprehensively analyze the advantages and disadvantages of each technique.Based on the fouling mechanisms of Limnoperna fortunei,environmental impacts, and actual situation of water diversion and transfer tunnels project, we put forward a multi-tiered and segmented management strategy that is suitable for improving the management efficiency while minimizing ecological disruption.This study provides a scientific foundation and practical guidance for biofouling prevention and control of water diversion and transfer tunnels project, ensuring their long-term operational stability.
Adsorption of Methylene Blue by Cellulose/Carboxymethyl Chitosan Composite Aerogel
YANG Bing;CHEN Ziyue;RAN Jianhua;YAN Chao;GUO Yi;Using cellulose and carboxymethyl chitosan(CMCS) as raw materials, NaOH-urea aqueous solution as a solvent, and epichlorohydrin as a crosslinking agent, we prepared cellulose/CMCS composite aerogel and used it for the adsorption of Methylene Blue(MB).Moreover, we investigated the influencing factors of adsorption efficiency, and analyzed the kinetics and thermodynamics of adsorption behavior.The results show that the adsorption equilibrium is achieved after 180 min for adsorption of 40 mL 100 mg·L-1 MB by 20 mg composite aerogel under the conditions as follows: the mass ratio of cellulose to CMCS of 5∶5,the temperature of 25 ℃,and the pH value of 7.The adsorption capacity of MB reaches 188.67 mg·g-1,and the removal rate reaches 94.33%.The composite aerogel has good recycling performance.After five times of adsorption-desorption, the adsorption capacity of MB is 92.80 mg·g-1,and the removal rate can still reach more than 90%.The adsorption behavior of the composite aerogel to MB conforms to the pseudo-second-order kinetic model and the Langmuir isothermal adsorption model.Cellulose/CMCS composite aerogel can be used as an efficient and renewable biomass-based adsorbent for the treatment of printing and dyeing wastewater.
Preparation, Characterization, and in vitro Transfection of Spermine-Grafted Hydroxyethyl Amylose
XIONG Xiaoqin;KANG Jiali;XIONG Qi;FANG Xuefei;Through grafting spermine(Spm) onto the backbone of hydroxyethyl amylose(HEA),we prepared Spm-g-HEA polymer, and characterized the structure of the polymer via FTIR,~1HNMR,and TNBS method.Moreover, based on the electrostatic adsorption of Spm-g-HEA and plasmid pEGFP-N1,we prepared Spm-g-HEA/pDNA complexes with different mass ratios, and analyzed the particle size, Zeta potential, morphology, and stability of the complexes via dynamic light scattering, electrophoretic light scattering, transmission electron microscopy, and gel retardation assay.Furthermore, we evaluated the transfection efficiency and cytotoxicity of Spm-g-HEA/pDNA complexes on HEK-293T cells via inverted fluorescence microscopy, flow cytometry, and MTT assay.The results show that, when the molar ratio of Spm to HEA is 2∶1,Spm will be grafted onto HEA backbond with a grafting degree of 3.6(based on 100 glucose units).Spm-g-HEA can tightly adsorb pDNA and compress it into positively charged irregular spherical nanoparticles at 100-200 nm.When Spm-g-HEA is complexed with pDNA at a mass ratio of 16∶1,it will exhibit the highest transfection efficiency and non-cytotoxicity, indicating that Spm-g-HEA is expected to develop into a safe and efficient non-viral transgenic vector.
Optimization in Extraction Process of Total Polyphenols from Rosa roxburghii Tratt Fruits by Response Surface Methodology and Its Antioxidant Activity
LIANG Huiqing;LI Qianqian;LIAO Yuxing;HUANG Hongmei;LI Xueying;CHEN Guiyuan;ZHANG Cuixiang;We extracted total polyphenols from wild Rosa roxburghii Tratt fruits in Guizhou Province by solvent extraction.Based on the single-factor experiments, we optimized the extraction process by response surface methodologies, and explored the antioxidant activity of total polyphenols from Rosa roxburghii Tratt fruits.The results show that the optimal extraction conditions are as follows: the extraction time of 3.5 h, the extraction temperature of 60 ℃,the solid-liquid ratio of 1∶30(g∶mL),and the ethanol volume fraction of 60%.Under above conditions, the extraction amount of total polyphenols from Rosa roxburghii Tratt fruits reaches(15.310 0±0.072 6) mg·g-1.The scavenging ability of the total polyphenols from Rosa roxburghii Tratt fruits on DPPH and ABTS free radicals is positively correlated with its concentration within a certain concentration range, which is higher than that of ascorbic acid at the same concentration.The total polyphenols from Rosa roxburghii Tratt fruits exhibits strong antioxidant activity and possesses potential as natural antioxidants or food additives.
Preparation and in vitro Dissolution of Imatinib Liposomes
LIU Chunyu;HU Xuelei;In response to the problems of poor solubility,low oral bioavailability,and adverse reactions caused by Imatinib,we prepared Imatinib liposomes by passive drug-loading and active drug-loading processes,respectively.Moreover,we optimized the process parameters through single-factor experiments by using the encapsulation efficiency as an indicator.Furthermore,we systematically studied the microscopic morphology,particle size,Zeta potential,in vitro release behavior,and stability of Imatinib liposomes.The results show that,in the passive drug-loading process, the encapsulation efficiency of Imatinib liposomes prepared by film dispersion method is the highest[(67.0±2.4)%];in the active drug-loading process,under the conditions of the drugloading time of 40 min,the drug-loading temperature of 50 ℃,the drug-lipid ratio of 1:30,the transmembrane pH gradient of 5,and the ammonium sulfate concentration of 100 mmol·L-1,the encapsulation efficiency of Imatinib liposomes is the highest[(65.0±4.1) %].The liposomes prepared by both two processes have regular morphology,spherical or nearly spherical shapes,with a particle size of 100-110 nm,showing good dispersion.The Imatinib liposomes prepared by the active drug-loading process exhibit good sustained-release effect in vitro and have better stability at 4 ℃ refrigeration than that at room temperature.The study lays a foundation for the development of a new drug delivery system for Imatinib.
[Downloads: 0 ] [Citations: 0 ] [Reads: 0 ] HTML PDF Cite this article
Efficient Adsorption of Glyphosate by Cerium Oxide-Modified Coconut Shell Biochar and Its Mechanism
LI Songyan;YI Xingguo;YU Shuilian;DENG Cuiwen;HUANG Yingping;HUANG Di;LIU Honglin;Using Ce(NO3)3 and coconut shell biochar(CBC) as raw materials,we prepared cerium oxidemodified coconut shell biochar(CO-CBC) via co-precipitation-high temperature calcination method,and characterized its structure by SEM,EDS,N2 adsorption-desorption isotherm,and pore size distribution curve.Moreover,we studied the adsorption performance of CO-CBC on Glyphosate by kinetic model and isothermal adsorption model,and systematically investigated the effects of CO-CBC dosage,p H value,and co-existent in water on the adsorption process.Furthermore,we revealed the adsorption mechanism by in situ FTIR and surface charge analysis.The results show that CO-CBC has a rough and porous morphology,and its adsorption capacity is 5.66 times that of CBC treated with the same calcination temperature(400-CBC).The adsorption of Glyphosate by CO-CBC is consistent with the pseudo-first-order,pseudo-second-order kinetic models and Freundlich isothermal adsorption model,and the adsorption process includes physical adsorption and multi-molecular layer chemisorption on non-uniform surfaces.CO-CBC binds to the carboxyl and amine groups of Glyphosate through the Ce sites,which is affected by charge effect.This study provides a new strategy for efficient removal of Glyphosate in water.
[Downloads: 405 ] [Citations: 0 ] [Reads: 0 ] HTML PDF Cite this article
Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[Downloads: 7,597 ] [Citations: 272 ] [Reads: 5 ] HTML PDF Cite this article
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research Progress on Pretreatment Technologies of Lignocellulose
ZHAO Lü1,LI Zhi-guang1,2,LI Hui-yong1,2,XIE Lei1, ZHOU Zhi1(1.College of Science,Hunan Agricultural University,Changsha 410128,China;2.Institute of Cheimstry & Chemical Engineering,Central South University,Changsha 410083,China)This paper reviews the components and structure of lignocellulose,states some promising pretreatment technologies in recent years,evaluates the main pretreatment technologies.
[Downloads: 3,006 ] [Citations: 209 ] [Reads: 4 ] HTML PDF Cite this article
Research Progress of Chlorogenic Acid
DENG Liang, YUAN Hua, YU Zong-yuan(Hubei Research Institute of Chemistry, Wuhan 430074, China)The distribution ,biosynthesis,properties, pharmaceutical activity,the extraction and purifying technology and applied prospect of chlorogenic acid were reviewed in this paper, which tried to provide references for the research of chlorogenic acid, and the production of the medicines comprising chlorogenic acids as well.
Research Advances of Separation and Purification for Protein
WANG Zi-jia1,LI Hong-mei2,GONG Ai-jun1,CAO Yan-qiu1,YUAN Hai-tao1(1.University of Science and Technology Beijing,Beijing 100083,China;2.National Institute of Metrology,Beijing 100013,China)The method of separation and purification for protein is one of the hotspots of the field for life science.This article summarizes many methods of separation and purification,based on the characteristic of protein′s acid-bases,the size and shape of the molecular,the solubility and the compatibility with legends.
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[Downloads: 7,597 ] [Citations: 272 ] [Reads: 5 ] HTML PDF Cite this article
Research Progress of Immobilized Enzyme
CHEN Jian-long~(1,2),QI Jian-cheng~1,CAO Yi-zhi~2,GUO Yong~1,DUAN Hui-li~1(1.Institute of Medical Equipment,Academy of Military Medical Sciences, National Engineering Research Center for Biological Protective Equipment,Tianjin 300161,China;2.School of Life Science,Lanzhou University,Lanzhou 730000,China)The immobilized enzymes have been widely applied in many fields for their more advantages than free enzyme,especially for their stability and reusability.At present,immobilization technologies of enzyme have obtained wonderful development.The research of immobilized enzyme will be a very important development field in the enzyme engineering in the future.In this article,various conventional and new methods for preparation of immobilized enzyme are introduced. At the same time,the immobilization of enzyme on some carriers with excellent properties are reviewed.