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Editorial Department of "Chemistry ＆ Bioengineering" has been Awarded "Advanced Collective for Studying and Implementing the Spirit of the Third Plenary Session of the 20th Central Committee of the Communist Party of China" by Hubei Provincial Editology Society for Science Periodicals in 2024
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Research Progress in Chemical Compositions and Pharmacological Effects of Eupatorium adenophorum Spreng.
GONG Ranran;DONG Fawu;GAO Lingxiao;WEI Yutong;ZHANG Qingwen;TIAN Yuan;WEI Shanshan;Eupatorium adenophorum Spreng.is a worldwide invasive weed that causes significant damage to the ecosystems and biodiversity of invaded areas.Modern pharmacological studies have shown that Eupatorium adenophorum Spreng.has antibacterial, antifungal, antiviral, anti-inflammatory, and anti-tumor effects.Therefore, the research approach towards Eupatorium adenophorum Spreng.has gradually shifted from eradication and control to comprehensive utilization.We review the research progress of Eupatorium adenophorum Spreng.in terms of chemical compositions(terpenoids, flavonoids, steroids, and phenylpropanoids),pharmacological effects, and toxicity.Moreover, we put forward prospects in the future research priorities.
Synthesis Progress in Norcembranoid Diterpenoids
ZENG Shanji;LI Di;LONG Ai;HE Shuzhong;The norcembranoid diterpenoid natural products possess a complex and compact tetracyclic core scaffold, consisting of a functionalized six-or seven-membered ring, along with four to eight adjacent stereogenic centers, and several unstable structural units.The norcembranoid diterpenoids exhibit biological activities such as anti-cancer, anti-inflammatory, and cytotoxicity.We summarize the synthesis progress of norcembranoid diterpenoid natural products in recent years, including scabrolide A,yonarolide, sinulochmodin C,and ineleganolide.
Research Progress in Biosynthesis of Phenyllactic Acid by Lactic Acid Bacteria and Its Production Improvement Strategies
ZHANG Guo;LI Wei;ZHOU Xi;LI Pei;LI Ku;CHEN Xiong;Lactic acid bacteria(LAB) are generally recognized as safe(GRAS) microorganisms.Their metabolites possess both functionality and safety, and they have the potential to produce high levels of phenyllactic acid(PLA).The biosynthesis method with LAB as the whole cell catalyst has become the mainstream technology direction due to its eco-friendliness and high product safety.However, the PLA synthesis ability of wild-type LAB is generally weak, making it difficult to meet the requirements of large-scale applications.Currently, improving the PLA production of LAB through biological and non-biological technologies has become a research hotspot.We introduce the metabolic pathway of PLA biosynthesis in LAB,and summarize strategies for increasing the PLA production of LAB from aspects such as the screening of high-yield strains, coenzymes regeneration, gene expression regulation, the application of new technologies(quorum sensing, immobilization technologies, co-culture of cells, and cryogel encapsulation, etc.),and fermentation process optimization.The study provides a reference for the green industrial production of PLA.
Chemotactic Performance of Glucose Oxidase-Driven Micromotors
SI Yanbing;GUAN Qiaoxin;XU Leilei;We prepared SiO2@Au-GOx Janus micromotors by sputtering a gold(Au) layer on the surface of SiO2 microspheres by using magnetron sputtering technique and loading with glucose oxidase(GOx).Moreover, we characterized the morphology and structure of the micromotors by field emission scanning electron microscope and inverted fluorescent microscope, and evaluated the motion performance.Furthermore, we investigated the chemotactic performance of the micromotors through microfluidic channel.The results show that SiO2@Au-GOx micromotors are formed by half-coating the Au layer on the surface of SiO2 microspheres to form a Janus structure and loading GOx on the opposite side, with a diameter of about 3 μm.When no glucose is added to the right side of the microfluidic channel, the motion range, displacement of mass center, and directionality of the micromotors all show a trend of first increasing and then decreasing with the increase of glucose concentration in the agar on the left side, and the best chemotactic performance is achieved at a concentration of 200 mmol·L-1.When the concentration gradient difference between the two sides of the microfluidic channel is the same, the micromotors show the same motion tendency with the increase of the initial glucose concentration on the right side, indicating that the size of the glucose concentration gradient field and the initial glucose concentration can affect the chemotactic performance of the micromotors.
Efficient Adsorption of Glyphosate by Cerium Oxide-Modified Coconut Shell Biochar and Its Mechanism
LI Songyan;YI Xingguo;YU Shuilian;DENG Cuiwen;HUANG Yingping;HUANG Di;LIU Honglin;Using Ce(NO3)3 and coconut shell biochar(CBC) as raw materials, we prepared cerium oxide-modified coconut shell biochar(CO-CBC) via co-precipitation-high-temperature calcination method, and characterized its structure by SEM,EDS,N2 adsorption-desorption isotherm, and pore size distribution curve.Moreover, we studied the adsorption performance of CO-CBC on Glyphosate by using the kinetic model and the isothermal adsorption model, and systematically investigated the effects of CO-CBC dosage, pH value, and co-existent in water on the adsorption process.Furthermore, we revealed the adsorption mechanism by in situ FTIR and surface charge analysis.The results show that CO-CBC has a rough and porous morphology, and its adsorption capacity is 5.66 times that of CBC treated with the same calcination temperature(400-CBC).The adsorption of Glyphosate by CO-CBC is consistent with the pseudo-first-order, pseudo-second-order kinetic models and Freundlich isothermal adsorption model, and the adsorption process includes physical adsorption and multi-molecular layer chemisorption on non-uniform surfaces.CO-CBC binds to the carboxyl and amine groups of Glyphosate through the Ce sites, which is affected by charge effect.This study provides a new strategy for efficient removal of Glyphosate in water.
Anti-Breast Cancer Active Components in Loquat Leaves and Their Inhibitory Effects on Topoisomerase Ⅰ
LIU Jiayao;YANG Junlong;XIE Yuman;HUANG Hui;ZENG Huang;NIE Hua;GUO Bohong;In order to explore the anti-breast cancer active components in loquat leaves and their inhibitory effects on topoisomerase Ⅰ(TOP Ⅰ),we integrated network pharmacology to screen the anti-breast cancer active components from loquat leaves and core pathways.Moreover,we analyzed the interaction mode and inhibitory efficacy of the active components with TOP Ⅰ by using molecular docking and TOP Ⅰ-mediated DNA relaxation experiments.Furthermore,we investigated the effects of the active components on the proliferation,migration,cloning,and apoptosis of human breast cancer cells MCF-7 by cytotoxicity experiments,cell scratch healing experiments,cell cloning experiments,and cell apoptosis experiments.The results show that quercetin,ellagic acid,(-)-epigallocatechin gallate,and kaempferol are the key anti-breast cancer active components in loquat leaves,they can stably bind to the catalytic domain of TOP Ⅰ.All four active components can inhibit the activity of TOP Ⅰ in a concentration-dependent manner.The half-maximal inhibitory concentrations(IC50) of the four active components are 160.70,84.82,72.54,and 80.67 μmol·L-1,respectively,significantly inhibiting the growth,proliferation,migration,and cloning of MCF-7 cells and promoting cell apoptosis.This study provides a theoretical basis for the research of antitumor activity of loquat leaves extracts.
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Research Progress in Carbon-Based Catalysts Modification Strategies and Electrochemical Reactor for Synthesis of H_2O2 via 2e-ORR
LU Zilan;JIA Daqing;HU Huawei;ZHU Leilei;KHAN Asim;XU Wenxuan;LIAO Benren;ZHANG Lehua;The synthesis of hydrogen peroxide(H_2O2) via two-electron oxygen reduction reaction(2e-ORR) has become a new and sustainable alternative to industrial anthraquinone process due to its advantages of normal temperature and pressure operation,green raw materials,and renewable energy.Based on the brief explanation of synthetic mechanism of H_2O2 via 2e-ORR,we review the modification strategies of carbon-based catalysts,including heteroatom doping,defect engineering,functional groups,etc.At the same time,we summarize the structure characteristics and application scenarios of H-type,flow-type,and membrane electrode assembly(MEA) reactors,and point out the challenges faced by the current technology,which provides a theoretical support for the research of H_2O2 synthesis via 2e-ORR.
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Preparation and in vitro Dissolution of Imatinib Liposomes
LIU Chunyu;HU Xuelei;In response to the problems of poor solubility,low oral bioavailability,and adverse reactions caused by Imatinib,we prepared Imatinib liposomes by passive drug-loading and active drug-loading processes,respectively.Moreover,we optimized the process parameters through single-factor experiments by using the encapsulation efficiency as an indicator.Furthermore,we systematically studied the microscopic morphology,particle size,Zeta potential,in vitro release behavior,and stability of Imatinib liposomes.The results show that,in the passive drug-loading process, the encapsulation efficiency of Imatinib liposomes prepared by film dispersion method is the highest[(67.0±2.4)%];in the active drug-loading process,under the conditions of the drugloading time of 40 min,the drug-loading temperature of 50 ℃,the drug-lipid ratio of 1:30,the transmembrane pH gradient of 5,and the ammonium sulfate concentration of 100 mmol·L-1,the encapsulation efficiency of Imatinib liposomes is the highest[(65.0±4.1) %].The liposomes prepared by both two processes have regular morphology,spherical or nearly spherical shapes,with a particle size of 100-110 nm,showing good dispersion.The Imatinib liposomes prepared by the active drug-loading process exhibit good sustained-release effect in vitro and have better stability at 4 ℃ refrigeration than that at room temperature.The study lays a foundation for the development of a new drug delivery system for Imatinib.
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Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[Downloads: 7,597 ] [Citations: 272 ] [Reads: 14 ] HTML PDF Cite this article
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research Progress on Pretreatment Technologies of Lignocellulose
ZHAO Lü1,LI Zhi-guang1,2,LI Hui-yong1,2,XIE Lei1, ZHOU Zhi1(1.College of Science,Hunan Agricultural University,Changsha 410128,China;2.Institute of Cheimstry & Chemical Engineering,Central South University,Changsha 410083,China)This paper reviews the components and structure of lignocellulose,states some promising pretreatment technologies in recent years,evaluates the main pretreatment technologies.
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Research Progress of Chlorogenic Acid
DENG Liang, YUAN Hua, YU Zong-yuan(Hubei Research Institute of Chemistry, Wuhan 430074, China)The distribution ,biosynthesis,properties, pharmaceutical activity,the extraction and purifying technology and applied prospect of chlorogenic acid were reviewed in this paper, which tried to provide references for the research of chlorogenic acid, and the production of the medicines comprising chlorogenic acids as well.
Research Advances of Separation and Purification for Protein
WANG Zi-jia1,LI Hong-mei2,GONG Ai-jun1,CAO Yan-qiu1,YUAN Hai-tao1(1.University of Science and Technology Beijing,Beijing 100083,China;2.National Institute of Metrology,Beijing 100013,China)The method of separation and purification for protein is one of the hotspots of the field for life science.This article summarizes many methods of separation and purification,based on the characteristic of protein′s acid-bases,the size and shape of the molecular,the solubility and the compatibility with legends.
[Downloads: 12,053 ] [Citations: 172 ] [Reads: 12 ] HTML PDF Cite this article
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[Downloads: 7,597 ] [Citations: 272 ] [Reads: 14 ] HTML PDF Cite this article
Research Progress of Immobilized Enzyme
CHEN Jian-long~(1,2),QI Jian-cheng~1,CAO Yi-zhi~2,GUO Yong~1,DUAN Hui-li~1(1.Institute of Medical Equipment,Academy of Military Medical Sciences, National Engineering Research Center for Biological Protective Equipment,Tianjin 300161,China;2.School of Life Science,Lanzhou University,Lanzhou 730000,China)The immobilized enzymes have been widely applied in many fields for their more advantages than free enzyme,especially for their stability and reusability.At present,immobilization technologies of enzyme have obtained wonderful development.The research of immobilized enzyme will be a very important development field in the enzyme engineering in the future.In this article,various conventional and new methods for preparation of immobilized enzyme are introduced. At the same time,the immobilization of enzyme on some carriers with excellent properties are reviewed.