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Editorial Department of "Chemistry ＆ Bioengineering" has been Awarded "Advanced Collective for Studying and Implementing the Spirit of the Third Plenary Session of the 20th Central Committee of the Communist Party of China" by Hubei Provincial Editology Society for Science Periodicals in 2024
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Research Progress in Antimicrobial Peptide Nanocomposites for Antibacterial Applications
WU Qijun;ZHANG Fan;HAN Yuxiao;DENG Shuyuan;WANG Bo;FU Jian;SHE Yuehui;We introduce the main types of antimicrobial peptide nanocomposites(AMP-NCs),including AMP-metal nanoparticles, AMP-self-assembled inorganic nanoparticles, AMP-polymer conjugates, and AMP-nanoenzymes, along with their synthesis methods.Moreover, we systematically elaborate on the antibacterial mechanisms of AMP-NCs, covering three aspects: disruption of cell membrane, interference with cellular metabolism, and inhibition of biofilm formation.Furthermore, we analyze the key factors influencing their antibacterial performance, and put forward current challenges of AMP-NCs for antibacterial applications and propspects in the future dovelopment directions.The review provides a theoretical foundation for developing next-generation AMP-NCs-based antibacterial systems targeting microbiologically influenced corrosion.
Research Progress in High-Density Fermentation Technology of Porcine Bacterial Pathogens
MA Yongkang;HAN Si'e;SU Xiaoming;LIU Lixia;LIU Zhanying;Inner Mongolia Key Laboratory of Synthetic Biology;Engineering Research Center for Green Manufacturing in Bio-Fermentation Industry;Inner Mongolia Specialized Technology Research and Pilot Public Service Platform for Biological Fermentation;Porcine bacterial pathogens pose a serious threat to the healthy development of the pig industry, and inactivated vaccines have become the primary prevention and control means.High-density fermentation technology is crucial for achieving large-scale, and efficient production of antigens.To address the issues such as low proliferation efficiency of bacteria and significant batch-to-batch antigen variability caused by insufficient nutrient adaptation, metabolic inhibition, and environmental instability during high-density fermentation, we review the optimization strategies for key factors including nutrient consumption patterns, culture conditions, and fermentation processes.Moreover, we systematically summarize the optimization strategies for various nutritional components such as carbon sources, nitrogen sources, inorganic salts, serum, and NAD cofactors, deeply analyze the mechanisms underlying critical culture conditions such as temperature, pH value, and dissolved oxygen, and summarize the characteristics and applications of fermentation processes under different feedback signals.Furthermore, we put forward key suggestions such as in-depth optimization of culture media, multi-faceted modification of strains, and multi-strain co-fermentation.The review provides solid theoretical supports and clear development directions for the industrial-scale production of inactivated vaccines, thereby promoting the healthy and sustainable development of the pig industry.
Research Progress of BODIPY Fluorescent Probes in Detection of Biothiols
WANG Tianshuai;ZHANG Kaiqi;ZHU Rongke;MA Chengjie;LI Zhiheng;Abnormal expression of biothiols such as cysteine(Cys),homocysteine(Hcy),and glutathione(GSH) is closely associated with various diseases, making highly sensitive and specific detection of these species of significant clinical importance.Boron dipyrromethene(BODIPY) fluorescent probes have emerged as a research hotspot in the field of biological detection due to their advantages including high molar absorption coefficient, high quantum yield, and ease of structural modification.We review the research progress of BODIPY fluorescent probes for biothiol detection over the past five years, and introduce fluorescent probes specifically responsive to individual biothiols(Cys or GSH) as well as multifunctional fluorescent probes capable of simultaneously identifying or discriminating among multiple biothiols(Cys, Hcy, and GSH).Moreover, we systematically discuss molecular design strategies, response mechanisms, photophysical properties, detection limits, and applications in live-cell imaging, in vivo imaging, and portable solid-state detection.Furthermore, we put forward the challenges and future development directions in this field.
Preparation of CVB-D@MIL-100(Fe) Drug-Loaded Composite and Determination of Its Drug Loading Capacity
ZHOU Huanhuan;CUI Tingting;ZHANG Haiwen;ZHANG Yi;ZHAO Rou;HAN Xiaoyan;We synthesized MIL-100(Fe) via a hydrothermal method, and prepared the drug-loaded composite CVB-D@MIL-100(Fe) by impregnating and adsorbing Cyclovirobuxinum D(CVB-D) onto MIL-100(Fe).Moreover, we characterized MIL-100(Fe) before and after drug loading by FTIR,Zeta potential, SEM,and XRD.Furthermore, we established a pre-column derivatization HPLC method to determine the drug loading capacity, and conducted its methodological validation.The results show that CVB-D is successfully loaded onto MIL-100(Fe) without altering its crystal structure or micro-morphology.The peak area exhibits a good linear relationship with CVB-D concentration in the range of 5.245-104.900 μg·mL-1(R2=0.999 8).The relative standard deviations for precision, repeatability, and stability are 1.35%,1.74%,and 0.66%,respectively, and the average recovery is(100.28±1.52)%.MIL-100(Fe) demonstrates high loading capacity for CVB-D with an average drug loading capacity of(24.41±0.64)% in CVB-D@MIL-100(Fe).The established pre-column derivatization HPLC method is simple, specific, and accurate, which is suitable for determining the drug loading capacity of CVB-D@MIL-100(Fe).The study provides a valuable reference for improving the bioavailability of CVB-D and developing novel formulations.
Electro-Fenton Performance of Graphite Felt Cathode Synergistically Modified by Electrodeposition
LIU Shanshan;YANG Yang;FENG Zexuan;HAN Yuyuan;LIU Jintian;DAI Jie;We prepared modified graphite felt(PPy-Mn-β-CD/GF) using β-cyclodextrin(β-CD),polypyrrole(PPy),and manganese sulfate(MnSO4).Moreover, we systematically investigated its degradation performance for dye wastewater as a cathode in the sodium disilicate-electro-Fenton(SD-EF) system.The results show that the loading of β-CD roughens the surface morphology of the GF substrate and increases the specific surface area, effectively preventing the aggregation of Mn particles.PPy and Mn are successfully loaded on the surface of GF,achieving co-doping of N and Mn.Compared to GF-0,the contact angle of PPy-Mn-β-CD/GF decreases from 129.1°to 102.9°, significantly enhancing hydrophilicity.When treating actual dye wastewater in the SD-EF system, PPy-Mn-β-CD/GF cathode demonstrates improvements of 55.1%,31.29%,and 5.83% in H_2O2 production, COD removal rate, and decolorization rate, respectively, compared to GF-0,while maintaining the energy consumption of 0.42 kW·h·(g COD)-1. After six cycles of reuse, H_2O2 production decreases by only 9.87%,indicating excellent stability.The PPy-Mn-β-CD electrodeposition modification of GF has potential application value.
Research Progress in Application of Functional Microorganisms and Their Composite Microbial Inoculants in Straw Composting
QIN Huifeng;LI Guangyu;ZHAO Chunxiao;HU Jianhua;LIU Zhanying;As an important way for efficient utilization of straw,composting efficiency of straw composting is closely related to the development and application of microbial inoculants.Straw contains high levels of cellulose,hemicellulose,and lignin,making it difficult to degrade,the rational use of microbial inoculants is crucial for improving composting efficiency.We review the roles of functional microorganisms such as Bacillus and Aspergillus in straw composting.Moreover,we introduce the degradation mechanisms of cellulases,hemicellulases,and ligninases.Furthermore,we summarize the screening and breeding methods of superior strains,and compare the formulations and application effects of various composite microbial inoculants.The study provides theoretical foundations and practical guidance for the further research and development,and large-scale application of microbial inoculants in straw composting.
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Ultrasonic-Assisted Deep Eutectic Solvent Extraction of Total Flavonoids from Nandina domestica Thunb. Fruits and Its in vitro Antioxidant and Antibacterial Activities
JIA Shanshan;REN Yuanyuan;CHEN Shengxi;PENG Yuan;We extracted total flavonoids from Nandina domestica Thunb.fruits by ultrasonic-assisted deep eutectic solvent method.Moreover,we optimized the extraction process through single-factor experiments and response surface methodologies,and studied the in vitro antioxidant and antibacterial activities.The results show that the optimal extraction conditions of total flavonoids from Nandina domestica Thunb.fruits are as follows:the ultrasonic time of 32 min,the solid-liquid ratio of 1:69(g:mL),the molar ratio of choline chloride to urea of 1:2.9,and the choline chloride-urea moisture content of 40%.Under above conditions, the yield of total flavonoids from Nandina domestica Thunb.fruits is 49.52 mg·g-1,which is close to the predicted value of 49.70 mg·g-1.The total flavonoids from Nandina domestica Thunb.fruits exhibits strong scavenging abilities against DPPH, ABTS,and hydroxyl free radicals,shows a certain antibacterial effect against Staphylococcus aureus,and can enhance the antibacterial effect of Ceftriaxone Sodium.
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Construction of An Indium-Modified Weakly Alkaline Aqueous Iron-Chromium Redox Flow Battery and Its Energy Storage Performance
LIU Yingying;ZHANG Xiaofei;LUO Jingyao;ZHU Zonghan;FENG Qinghao;LIU Xueyi;LI Yafei;XIE Xiaoyin;DENG Xiangyi;We prepared a CrPDTA chelate by the chelation reaction of PDTA with chromium ion,obtained an indium-modified graphite felt electrode by modifying graphite felt electrode with indium chloride, and characterized the CrPDTA chelate and indium-modified graphite felt electrode by FTIR,UV-Vis,SEM,and EDS.Moreover,we assembled an indium-modified weakly alkaline aqueous iron-chromium redox flow battery(InICRFB) by using indium-modified graphite felt electrode,K4 Fe(CN)6 as a positive electrolyte and CrPDTA as a negative electrolyte,and systematically investigated the effects of indium-modified graphite felt electrode on the key performance indicators of the battery,such as energy efficiency,voltage efficiency,and capacity retention rate,etc.Furthermore,we preliminarily explored the internal mechanism of the performance enhancement.The results show that there is a chelation between PDTA and chromium ions,and indium is uniformly attached to the surface of graphite felt electrode.Indium plays a key role in improving the electrode conductivity and reducing charge transfer losses.Compared with the pristine ICRFB,In-ICRFB has the better battery performance:after 200 charge-discharge cycles at a current density of 50 mA·cm-2,the Coulomb efficiency is about 99.0%,the energy efficiency is 81.4%,the voltage efficiency is 82.2 %,the capacity retention rate is 35.0 %,and the average capacity degradation rate of single cycle is 0.325%.
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Anti-Breast Cancer Active Components in Loquat Leaves and Their Inhibitory Effects on Topoisomerase Ⅰ
LIU Jiayao;YANG Junlong;XIE Yuman;HUANG Hui;ZENG Huang;NIE Hua;GUO Bohong;In order to explore the anti-breast cancer active components in loquat leaves and their inhibitory effects on topoisomerase Ⅰ(TOP Ⅰ),we integrated network pharmacology to screen the anti-breast cancer active components from loquat leaves and core pathways.Moreover,we analyzed the interaction mode and inhibitory efficacy of the active components with TOP Ⅰ by using molecular docking and TOP Ⅰ-mediated DNA relaxation experiments.Furthermore,we investigated the effects of the active components on the proliferation,migration,cloning,and apoptosis of human breast cancer cells MCF-7 by cytotoxicity experiments,cell scratch healing experiments,cell cloning experiments,and cell apoptosis experiments.The results show that quercetin,ellagic acid,(-)-epigallocatechin gallate,and kaempferol are the key anti-breast cancer active components in loquat leaves,they can stably bind to the catalytic domain of TOP Ⅰ.All four active components can inhibit the activity of TOP Ⅰ in a concentration-dependent manner.The half-maximal inhibitory concentrations(IC50) of the four active components are 160.70,84.82,72.54,and 80.67 μmol·L-1,respectively,significantly inhibiting the growth,proliferation,migration,and cloning of MCF-7 cells and promoting cell apoptosis.This study provides a theoretical basis for the research of antitumor activity of loquat leaves extracts.
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Research Progress in Carbon-Based Catalysts Modification Strategies and Electrochemical Reactor for Synthesis of H_2O2 via 2e-ORR
LU Zilan;JIA Daqing;HU Huawei;ZHU Leilei;KHAN Asim;XU Wenxuan;LIAO Benren;ZHANG Lehua;The synthesis of hydrogen peroxide(H_2O2) via two-electron oxygen reduction reaction(2e-ORR) has become a new and sustainable alternative to industrial anthraquinone process due to its advantages of normal temperature and pressure operation,green raw materials,and renewable energy.Based on the brief explanation of synthetic mechanism of H_2O2 via 2e-ORR,we review the modification strategies of carbon-based catalysts,including heteroatom doping,defect engineering,functional groups,etc.At the same time,we summarize the structure characteristics and application scenarios of H-type,flow-type,and membrane electrode assembly(MEA) reactors,and point out the challenges faced by the current technology,which provides a theoretical support for the research of H_2O2 synthesis via 2e-ORR.
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Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[Downloads: 7,597 ] [Citations: 272 ] [Reads: 32 ] HTML PDF Cite this article
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research Progress on Pretreatment Technologies of Lignocellulose
ZHAO Lü1,LI Zhi-guang1,2,LI Hui-yong1,2,XIE Lei1, ZHOU Zhi1(1.College of Science,Hunan Agricultural University,Changsha 410128,China;2.Institute of Cheimstry & Chemical Engineering,Central South University,Changsha 410083,China)This paper reviews the components and structure of lignocellulose,states some promising pretreatment technologies in recent years,evaluates the main pretreatment technologies.
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Research Progress of Chlorogenic Acid
DENG Liang, YUAN Hua, YU Zong-yuan(Hubei Research Institute of Chemistry, Wuhan 430074, China)The distribution ,biosynthesis,properties, pharmaceutical activity,the extraction and purifying technology and applied prospect of chlorogenic acid were reviewed in this paper, which tried to provide references for the research of chlorogenic acid, and the production of the medicines comprising chlorogenic acids as well.
Research Advances of Separation and Purification for Protein
WANG Zi-jia1,LI Hong-mei2,GONG Ai-jun1,CAO Yan-qiu1,YUAN Hai-tao1(1.University of Science and Technology Beijing,Beijing 100083,China;2.National Institute of Metrology,Beijing 100013,China)The method of separation and purification for protein is one of the hotspots of the field for life science.This article summarizes many methods of separation and purification,based on the characteristic of protein′s acid-bases,the size and shape of the molecular,the solubility and the compatibility with legends.
[Downloads: 12,053 ] [Citations: 172 ] [Reads: 30 ] HTML PDF Cite this article
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[Downloads: 7,597 ] [Citations: 272 ] [Reads: 32 ] HTML PDF Cite this article
Research Progress of Immobilized Enzyme
CHEN Jian-long~(1,2),QI Jian-cheng~1,CAO Yi-zhi~2,GUO Yong~1,DUAN Hui-li~1(1.Institute of Medical Equipment,Academy of Military Medical Sciences, National Engineering Research Center for Biological Protective Equipment,Tianjin 300161,China;2.School of Life Science,Lanzhou University,Lanzhou 730000,China)The immobilized enzymes have been widely applied in many fields for their more advantages than free enzyme,especially for their stability and reusability.At present,immobilization technologies of enzyme have obtained wonderful development.The research of immobilized enzyme will be a very important development field in the enzyme engineering in the future.In this article,various conventional and new methods for preparation of immobilized enzyme are introduced. At the same time,the immobilization of enzyme on some carriers with excellent properties are reviewed.