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The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
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Basic Information:
China Classification Code:R151
Citation Information:
[1]MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China).Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry[J].Chemistry ＆ Bioengineering,2012,29(08):92-94.
2012-08-25
2012-08-25