被引排行
Research and Application Progress of Catalase
LIU Ling-zhi1,2,ZHONG Guang-rong1,XIONG Lian1,2,CHANG Yan-hong2,XIAO Bao-qing2,LUO Hui1(1.Department of Biological Science and Technology,University of Science and Technology Beijing,Beijing 100083,China;2.Department of Environmental Engineering,University of Science and Technology Beijing,Beijing 100083,China)As one kind of oxidase,catalase can be found in almost all animals,plants and microorganisms.With high efficiency in peroxide catalyzing,catalases have important potential in industrial applications.On the base of reviewing enzyme classification,functions and structure,this paper focuses on the enzyme purifying and the research progress of catalases from extremophiles,and introduces the applications of catalase on food industry,environmental protection,paper making industry and textile industry.
Comparison and Improvement of Three Determination Methods for Lipase Activity
JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China)Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
Determination of Vitamin C Content in Five Kinds of Fruits and Vegetables by UV Spectrophotometry
MA Hong-fei,LU Sheng-you,HAN Qiu-ju,LI Wei(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001,China)The vitamin C contents in five familiar fruits and vegetables were determined by UV spectrophotometry on the basis of the vitamin C characters of UV absorbing and alkali instability.The maximum absorbing wavelength of vitamin C determination by UV spectrophotometry was 243.4 nm,the standard curve equation was A=0.0528c-0.0568,the correlation coefficient R2=0.9996.The vitamin C content in green pepper,citrus junos,cucumber,apple,tomato was 117.1 mg·(100 g)-1,69.3 mg·(100 g)-1,20.5 mg·(100 g)-1,14.9 mg·(100 g)-1 and 18.4 mg·(100 g)-1,respectively.The determination of vitamin C content by UV spectrophotometric method was simple and fast with satisfactory result.
Research Progress on Pretreatment Technologies of Lignocellulose
ZHAO Lü1,LI Zhi-guang1,2,LI Hui-yong1,2,XIE Lei1, ZHOU Zhi1(1.College of Science,Hunan Agricultural University,Changsha 410128,China;2.Institute of Cheimstry & Chemical Engineering,Central South University,Changsha 410083,China)This paper reviews the components and structure of lignocellulose,states some promising pretreatment technologies in recent years,evaluates the main pretreatment technologies.
Research Progress of Chlorogenic Acid
DENG Liang, YUAN Hua, YU Zong-yuan(Hubei Research Institute of Chemistry, Wuhan 430074, China)The distribution ,biosynthesis,properties, pharmaceutical activity,the extraction and purifying technology and applied prospect of chlorogenic acid were reviewed in this paper, which tried to provide references for the research of chlorogenic acid, and the production of the medicines comprising chlorogenic acids as well.
Research Progress in Remediation Technology of Heavy Metal Contaminated Soil
WANG Xingli;WANG Chenye;WU Xiaochen;WANG Jingbo;MU Xiaodong;YANG Xiaoshu;HU Xiaofei;GAO Jing;Hainan Institute of Environmental Sciences;With the rapid development of industry and agriculture,China is facing a severe situation of soil heavy metal pollution.The remediation of heavy metal contaminated soil has become an important issue in the field of ecological environment,which has attracted extensive attention.On the basis of the frontier of remediation technology for heavy metal contaminated soil at home and abroad,we shortly introduce the main sources of soil heavy metal pollution.Meanwhile,we expound and analyze the main remediation technologies of heavy metal contaminated soil in detail,such as physical remediation,chemical remediation,and biological remediation,etc.Aiming at the characteristics and disadvantages of various remediation technologies,we put forward the improvement and development directions of remediation technologies for heavy metal contaminated soil in the future,which provide a reference for the research of soil remediation.
Research Progress of Immobilized Enzyme
CHEN Jian-long~(1,2),QI Jian-cheng~1,CAO Yi-zhi~2,GUO Yong~1,DUAN Hui-li~1(1.Institute of Medical Equipment,Academy of Military Medical Sciences, National Engineering Research Center for Biological Protective Equipment,Tianjin 300161,China;2.School of Life Science,Lanzhou University,Lanzhou 730000,China)The immobilized enzymes have been widely applied in many fields for their more advantages than free enzyme,especially for their stability and reusability.At present,immobilization technologies of enzyme have obtained wonderful development.The research of immobilized enzyme will be a very important development field in the enzyme engineering in the future.In this article,various conventional and new methods for preparation of immobilized enzyme are introduced. At the same time,the immobilization of enzyme on some carriers with excellent properties are reviewed.
Study on the Antibacterial Activity of Quercetin
QIN Xiao-rong1,ZHANG Ming-jin1,2,GAO Xu-na1,LIN Yi1,MA Li1,HE Si-yi1 (1.College of Chemical Engineering and Technology,Wuhan University of Science and Technology,Wuhan 430081,China;2.Wuhan Institute of Physics and Mathematics,The Chinese Academy of Sciences,Wuhan 430071,China)The antibacterial activities of quercetin to bacteria,such as Escherichia coli,Staphylococcus aureus were preliminarily tested by Neo-Sensitab disk-diffusion method.The antibacterial activity to Staphylococcus aureas was the most remarkably,minimal bactericidal concentration(MBC) was less than 0.0061 μmol·mL-1.The antibacterial activity to Bacillus mucilaginosus was the secondly remarkably,minimal inhibitory concentration(MIC) was less than 0.0061 μmol·mL-1.Quercetin had obviously antibacterial activities to Escherichia coli,Bacillus thuringiensis,Bacillus subtilis,Pseudomonas aeruginosa,too.MIC was respectively 0.0242 μmol·mL-1,0.0061 μmol·mL-1,0.0242 μmol·mL-1,0.0121 μmol·mL-1.MBC was respectively 1.5522 μmol·mL-1,6.2086 μmol·mL-1,3.1043 μmol·mL-1,1.5522 μmol·mL-1.However,quercetin had no antibacterial activity to Ochrobactrum anthropi.Quercetin showed extensive antibacterial activity against Gram-negative bacteria and even more strongly than against Gram-positive bacteria.
Research Advances of Separation and Purification for Protein
WANG Zi-jia1,LI Hong-mei2,GONG Ai-jun1,CAO Yan-qiu1,YUAN Hai-tao1(1.University of Science and Technology Beijing,Beijing 100083,China;2.National Institute of Metrology,Beijing 100013,China)The method of separation and purification for protein is one of the hotspots of the field for life science.This article summarizes many methods of separation and purification,based on the characteristic of protein′s acid-bases,the size and shape of the molecular,the solubility and the compatibility with legends.
Application of Membrane Separation Technology in Treatment of Waste Water Containing Heavy Metal Ions
XIE Hui-ling, YE Hong-qi, ZENG Jian-xian(College of Chemistry and Chemical Engineering,Central South University, Changsha 410083, China)The fundamental principles and characteristics of heavy metal wastewater treatment using membrane separation technology were introduced, and the studies and applications of the electrodialysis, liquid membrane, nanofiltration, ultra-low-pressure reverse osmosis, micellar-enhanced ultrafiltration and polymer enhanced ultrafiltration were reviewed. The existing problems and further development were also analyzed and prospected.