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Three common lipase activity determination methods including alkaly titrition method,p-nitrophenol(NP) method and copper soap method,aiming at consuming time,accuracy and repeatability,are compared.With an effective way to terminate the reaction being found,the improved p-NP method is stable and rapid.With toluene instead of benzene,the improved copper soap method possesses less toxicity,good stability and repeatability.
[1]Reetz Manfred T.Lipases as practical biocatalysts[J].Current O-pinion in Chemical Biology,2002,6(2):145-150.
[2]Hasan Fariha,Shah Aamer Ali,Hameed Abdul.Industrial applica-tions of microbial lipases[J].Enzyme and Microbial Technology,2006,39(2):235-251.
[3]Brockman H L.Triglyceride lipase from porcine pancreas[J].Methods Enzymol,1981,71:619-627.
[4]何耀强,王炳武,谭天伟.假丝酵母99-125脂肪酶的发酵工艺研究[J].生物工程学报,2004,20(6):918-921.
[5]Vorderwuelbecke T,Kieslich K,Erdmann H.Comparison of lipa-ses by different assays[J].Enzyme Microb Technol,1992,14(8):631-639.
[6]Suznki T,Nakayama T,Kurihara,et al.A cold-active esterasewith a substrate preference for vinyl esters from a psychrotroph,Acinetobactersp.strain No.6:Gene cloning,purification,andcharacterization[J].J Mol Catal B Enzymatic,2002,16(5-6):255-263.
[7]Duncombe W G.The colorimetric micro-determination of longc-hain fatty acids[J].Biochem J,1963,88(1):7-10.
[8]纪建业.脂肪酶活力测定方法的改进[J].通化师范学院学报,2005,26(6):51-53.
[9]Schmidt D C,Sztajer H,Stoecklein W,et al.Screening,purificationand properties of a thermophilic lipase fromBacillus thermocaten-ulatus[J].Biochim Biophys Acta,1994,1214(1):43-53.
[10]Han Soo-Jin,Chung Ji Hyung,Cheong Chan Seong,et al.En-hancement of lipase activity fromAcinetobacterspecies SY-01 byrandom mutagenesis and the role of lipase-specific chaperone[J].Enzyme and Microbial Technology,2004,35(5):377-384.
Basic Information:
China Classification Code:TQ920.1
Citation Information:
[1]JIANG Hui-fang,WANG Ya-qin,LIU Chun-guo(Department of Biochemical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China).Comparison and Improvement of Three Determination Methods for Lipase Activity[J].Chemistry ＆ Bioengineering,2007,No.127(08):72-75.
2007-08-25
2007-08-25