SUN Shanshan1
ZHANG Fan4
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We conducted anaerobic cultivation of Bacillus subtilis(BSU) isolated from water samples of Qinghai Oilfield by using sodium formate as the electron donor to reduce Pd2+ to Pd0,and prepared palladium nanoparticles.Moreover, we systematically characterized the palladium nanoparticles by ultraviolet-visible spectroscopy(UV-Vis),transmission electron microscopy(TEM),energy dispersive spectroscopy(EDS),X-ray diffraction(XRD),and X-ray photoelectron spectroscopy(XPS).Furthermore, we preliminarily explored the formation mechanism of palladium nanoparticles by BSU.The results show that the prepared palladium nanoparticles are nearly spherical, with uniform particle size distribution, and the average particle size is smaller under neutral conditions(pH=7),being(4.01±1.80) nm.Pd, P,and S coexist in the palladium nanoparticles, indicating that the cell structure of BSU plays a synergistic role in the particle formation process.The palladium nanoparticles have a typical face-centered cubic(FCC) structure, and the main form of Pd element in the nanoparticles is Pd0,with a relative content of approximately 89.30%.It is speculated that the cell structure and metabolic process of BSU play a key role in the adsorption, reduction, and nuclear stabilization of Pd2+.The study provides a theoretical basis for the green biological synthesis of noble metal nanoparticles.
[1] JOKAR S M,FAROKHNIA A,GONÇALVES M C,et al.Hydrogen production from refinery outputs utilizing palladium membrane reactors:a review[J].International Journal of Hydrogen Energy,2025,136:229-251.
[2] LIU Q,ZHAO L.Low valent palladium clusters:synthesis,structures and catalytic applications[J].Chinese Journal of Chemistry,2020,38(12):1897-1908.
[3] EGAN-MORRISS C,KIMBER R L,POWELL N A,et al.Biotechnological synthesis of Pd-based nanoparticle catalysts[J].Nanoscale Advances,2022,4(3):654-679.
[4] HUYNH K H,PHAM X H,KIM J,et al.Synthesis,properties,and biological applications of metallic alloy nanoparticles[J].International Journal of Molecular Sciences,2020,21(14):5174.
[5] LIANG Y Y,DEMIR H,WU Y J,et al.Facile synthesis of biogenic palladium nanoparticles using biomass strategy and application as photocatalyst degradation for textile dye pollutants and their in-vitro antimicrobial activity[J].Chemosphere,2022,306:135518.
[6] SALUNKE B K,SAWANT S S,LEE S I,et al.Microorganisms as efficient biosystem for the synthesis of metal nanoparticles:current scenario and future possibilities[J].World Journal of Microbiology & Biotechnology,2016,32(5):88.
[7] NORDMEIER A,MERWIN A,ROEPER D F,et al.Microbial synthesis of metallic molybdenum nanoparticles[J].Chemosphere,2018,203:521-525.
[8] NARAYANAN K B,SAKTHIVEL N.Biological synthesis of metal nanoparticles by microbes[J].Advances in Colloid and Interface Science,2010,156(1/2):1-13.
[9] KATO Y,SUZUKI M.Synthesis of metal nanoparticles by microorganisms[J].Crystals,2020,10(7):589.
[10] 陈圆.生物纳米钯的制备、毒性及催化降解三氯生的研究[D].广州：华南理工大学，2020.CHEN Y.The study on the reduction and toxicity of Pd(Ⅱ) by microorganisms and catalytic degradation of triclosan by Pd(0)-NPs[D].Guangzhou:South China University of Technology,2020.
[11] SUNDARAM P A,AUGUSTINE R,KANNAN M.Extracellular biosynthesis of iron oxide nanoparticles by Bacillus subtilis strains isolated from rhizosphere soil[J].Biotechnology and Bioprocess Engineering,2012,17(4):835-840.
[12] SHETA B,EL-ZAHED M,NAWAREG M,et al.Nanoremediation of tilapia fish culture using iron oxide nanoparticles biosynthesized by Bacillus subtilis and immobilized in a free-floating macroporous cryogel[J].BMC Veterinary Research,2024,20(1):455.
[13] YARZA P,YILMAZ P,PRUESSE E,et al.Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences[J].Nature Reviews Microbiology,2014,12(9):635-645.
[14] MARUYAMA T,MATSUSHITA H,SHIMADA Y,et al.Proteins and protein-rich biomass as environmentally friendly adsorbents selective for precious metal ions[J].Environmental Science & Technology,2007,41(4):1359-1364.
[15] SAHU A K,DAS A,GHOSH A,et al.Understanding blue shift of the longitudinal surface plasmon resonance during growth of gold nanorods[J].Nano Express,2021,2(1):010009.
[16] PALOMBA S,NOVOTNY L,PALMER R E.Blue-shifted plasmon resonance of individual size-selected gold nanoparticles[J].Optics Communications,2008,281(3):480-483.
[17] BEVERIDGE T J,MURRAY R G.Sites of metal deposition in the cell wall of Bacillus subtilis[J].Journal of Bacteriology,1980,141(2):876-887.
[18] DOYLE R J,MATTHEWS T H,STREIPS U N.Chemical basis for selectivity of metal ions by the Bacillus subtilis cell wall[J].Journal of Bacteriology,1980,143(1):471-480.
[19] SOUTHAM G,BEVERIDGE T J.The occurrence of sulfur and phosphorus within bacterially derived crystalline and pseudocrystalline octahedral gold formed in vitro[J].Geochimica et Cosmochimica Acta,1996,60(22):4369-4376.
[20] PIAO Y Z,JANG Y J,SHOKOUHIMEHR M,et al.Facile aqueous-phase synthesis of uniform palladium nanoparticles of various shapes and sizes[J].Small,2007,3(2):255-260.
[21] HOLZWARTH U,GIBSON N.The Scherrer equation versus the "Debye-Scherrer equation"[J].Nature Nanotechnology,2011,6(9):534.
[22] SHAIK M R,ALI Z J Q,KHAN M,et al.Green synthesis and characterization of palladium nanoparticles using Origanum vulgare L.extract and their catalytic activity[J].Molecules,2017,22(1):165.
[23] WALDRON K J,ROBINSON N J.How do bacterial cells ensure that metalloproteins get the correct metal?[J].Nature Reviews Microbiology,2009,7(1):25-35.
[24] MAHENDRAN A,ORLANDO B J.Genome wide structural prediction of ABC transporter systems in Bacillus subtilis[J].Frontiers in Microbiology,2024,15:1469915.
[25] PINSKE C,SARGENT F.Exploring the directionality of Escherichia coli formate hydrogenlyase:a membrane-bound enzyme capable of fixing carbon dioxide to organic acid[J].Microbiology Open,2016,5(5):721-737.
[26] LAW C K Y,BONIN L,de GUSSEME B,et al.Biogenic synthesis of palladium nanoparticles:new production methods and applications[J].Nanotechnology Reviews,2022,11(1):3104-3124.
[27] 程峰，魏澜，王成娇，等.甲酸脱氢酶及其在手性生物制造中的应用[J].生物工程学报，2022,38(2):632-649.CHENG F,WEI L,WANG C J,et al.Formate dehydrogenase and its application in biomanufacturing of chiral chemicals[J].Chinese Journal of Biotechnology,2022,38(2):632-649.
[28] 胡玉婷.枯草芽孢杆菌不锈钢微生物腐蚀的胞外双向电子传递机制研究[D].北京：北京科技大学，2023.HU Y T.Study on microbiologically influenced corrosion of stainless steels by Bacillus subtilis via bidirectional extracellular electron transfer[D].Beijing:University of Science and Technology Beijing,2023.
[29] DUNDAS C M,GRAHAM A J,ROMANOVICZ D K,et al.Extracellular electron transfer by Shewanella oneidensis controls palladium nanoparticle phenotype[J].ACS Synthetic Biology,2018,7(12):2726-2736.
[30] 陈立香，李祎頔，田晓春，等.革兰氏阳性电活性菌的电子传递及其应用[J].化学进展，2020,32(10):1557-1563.CHEN L X,LI Y D,TIAN X C,et al.Electron transfer in Gram-positive electroactive bacteria and its application[J].Progress in Chemistry,2020,32(10):1557-1563.
Basic Information:
China Classification Code:TB383.1;TQ138.23
Citation Information:
[1]KOU Jingyi,SHEN Jing,LIAO Yue ,et al.Preparation of Palladium Nanoparticles Using Bacillus subtilis and Pd~(2+) Reduction Mechanism[J].Chemistry ＆ Bioengineering,2026,43(05):16-22.
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国家自然科学基金项目(52474053,52474028)
2025-11-28
2025-11-28
2025-11-28