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Taking the extraction rate of polysaccharides from Astragalus-Radix Puerariae herb pair as an evaluation index, we optimized the ultrasonic extraction process of polysaccharides from Astragalus-Radix Puerariae herb pair by response surface methodologies on the basis of single-factor experiments, and evaluated its antioxidant activity by the determination of scavenging rates of hydroxyl free radicals, DPPH free radicals, and superoxide anion free radicals.The results show that the optimal ultrasonic extraction process can be determined as follows: the liquid-solid ratio of 17∶1(mL∶g),the extraction time of 30 min, and the extraction temperature of 57 ℃.Under above conditions, the extraction rate of polysaccharides from Astragalus-Radix Puerariae herb pair can reach 13.78%.Polysaccharides from Astragalus-Radix Puerariae herb pair has certain antioxidant activity, and the deproteinization of polysaccharides can effectively improve its antioxidant activity.
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Basic Information:
China Classification Code:R284.2
Citation Information:
[1]YAN Hao,CHENG Fan,YAN Furong ,et al.Optimization in Ultrasonic Extraction Process of Polysaccharides from Astragalus-Radix Puerariae Herb Pair by Response Surface Methodology and Its Antioxidant Activity[J].Chemistry ＆ Bioengineering,2023,40(05):38-44.
Fund Information:
中国博士后科学基金项目(2021M690474); 陕西省重点研发计划项目(2023-YBSF-278); 咸阳市重点研发计划项目(L2022ZDYFSF054); 陕西省大学生创新创业训练计划项目(S202210716047)
2023-05-09
2023-05-09