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Application of Enzyme Engineering in Medicine Industry
CHEN Hong-xia (Department of Biology and Chemical Engineering ,Jining Vocational Technology College ,Jining 272037, China)Enzyme engineering is an important composing part of modern biotechnologies, it will take great effect in industry as a new technology. The new advance in enzyme engineering research includes such as enzyme immobilization,immobilization of genetically engineered microorganism(cell). enzyme production by plant cell culture,chemical modification of enzymes, ribozyme, abzyme, enzyme labelling drug and the application of enzyme engineering in the medicine industry. The prospects of enzyme engineering are also explored.
Progress on Synthesis and Application of Polycaprolactone
WANG Yong-liang,YI Guo-bin,KANG Zheng,XIONG Fu-hua,ZHOU Ping,CUI Yi-hua(Faculty of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,China)The properties,synthesis and application of polycaprolactone are reviewed.Main initiation systems,auxiliary means for the ring-opening polymerization of ε-caprolactone to obtain polycaprolactone and their application as biomedical materials are focused on and the developing direction is respected.
Refining of Dimethyl Sulfoxide by Vacuum Distillation
HU Jie;CHEN Feiyue;LI Dongfeng;WU Xiaofeng;Using the spinning waste liquid in the production of PAN-based carbon fibers as a raw material,we comparatively studied the changes of temperature and dimethyl sulfoxide(DMSO) concentration of each tower section when two technological processes such as the batch vacuum distillation and the batch distillation water at atmospheric pressure followed by vacuum distillation process were adopted,and determined the DMSO concentration in distillate by gas chromatography.The results show that,in the technological process of batch vacuum distillation,when the DMSO concentration in the tower bottom is 81% and the temperature of the first tower section is 77.1 ℃,the distillate begins to flow out,and the DMSO concentration in the distillate is up to 41%;in the technological process of batch distillation water at atmospheric pressure followed by vacuum distillation,when the temperature of the first tower section is 98.5 ℃,the distillate begins to flow out,and the DMSO concentration in the distillate is 1%;with the increase of time,the temperature of the first tower section increases and tends to be stable 100 ℃,the DMSO concentration in the tower top increases and tends to be stable 98%,at this point,the DMSO concentration in tower bottom is 100%,and the DMSO concentration in the distillate is as high as 100%.The study indicates that DMSO can be recovered from spinning waste liquid by the technological process of batch distillation water at atmospheric pressure followed by vacuum distillation.
Application Development of Aqueous Two-Phase Extraction Technology
FAN Fang(Department of Chemical and Biological Sciences,Guangdong University of Petrochemical Technology,Maoming 525000,China)This paper describes the basic characters and applications of aqueous two-phase extraction technology,including in biology engineering,extraction and separation of pharmaceutical components,separation and analysis of toxic substances in the environment,and combination with other technologies in the last three years.The prospects of aqueous two-phase extraction technology are discussed at the end of the paper.
Study on Partition and Purification of Protein by Aqueous Two-phase Technology
ZHENG Nan,LIU Jie(College of Environmental Science and Engineering,Nanchang University,Nanchang 330029,China)The fundamental extraction mechanism of aqueous two-phase system is explained.And the factors which influence partition and purification of protein are researched in detail.In the end,The application and expectation of aqueous two-phase technology in protein partition and purification are discussed.
Determination of Total Flavonoids and Total Polyphenols in Abelmoschi corolla Extracts and Their in vitro Antibacterial and Antioxidant Activities
SUN Zenghong;ZHANG Sha;WANG Liwa;GONG Haiyan;TIAN Shuge;In this paper, we determined the contents of total flavonoids and total polyphenols in Abelmoschi corolla extracts(ACE) from different origins by colorimetry.Moreover, we studied antibacterial activity in vitro of ACE by agar punching method and microdilution method.Furthermore, we evaluated the antioxidant activity of ACE by DPPH,ABTS,and FRAP methods.The results show that, there is a negligible difference in the contents of total flavonoids and total polyphenols in ACE from different origins.The content of total flavonoids in ACE from Anhui province is the highest at(4.68±0.37) mg·g-1,while the content of total polyphenols in ACE from Shaanxi province is the highest at(1.48±0.11) mg·g-1.ACE has certain antibacterial activity in vitro and can significantly inhibit the growth of Streptrococcus mutans in a concentration dependent manner.The minimum inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) are 3 mg·mL-1 and 6 mg·mL-1,respectively.ACE has certain antioxidant activity and can scavenge free radicals such as DPPH and ABTS,with a dose-response relationship within a certain concentration range.
Research and Application Progress in Cellulase
ZHAO Xin;ZHANG Hong;MEN Zhonghua;MA Yong;WEN Tong;Cellulose is mainly degraded by cellulase with different functions into oligosaccharides or monosaccharides, which provides carbon source and energy for herbivorous animals and some microorganisms, and plays an important role in the natural carbon cycle.In this paper, on the basis of the introduction of cellulose and cellulase, we review the research progress of cellulase and its applications in textile, paper making, feed, food, and energy industries.
Surface Modification of Biomedical Polymeric Materials
YANG Ying1,LI Qin2,ZHANG Han1,LIANG Fei1(1.Xi′an Medical University,Xi′an 710021,China;2.Shanxi Tumor Hospital,Xi′an 710061,China)Biocompatibility is the key problem to be resolved in the application of biomedical polymeric materials.Surface modification to improve the biocompatibility of materials have caused extensive concern.There are varieties of methods about surface modification,the methods from physics,chemistry,bionics three aspects were reviewed respectively in the paper.
Simulation and Optimization in Wastewater Recovery of 1,4-Butanediol by Aspen Plus Software
LI Qingpeng;YAO Yuanhong;ZHANG Xu;We treated 1,4-butanediol(BDO) wastewater by the combination process of scraper film evaporator and distillation column,simulated and optimized the process parameters by the Aspen Plus software.Moreover,we verified the feasibility of the simulated process conditions by intermittent decompression distillation experiments.The optimum process conditions are determined as follows:the feeding position is the 22 nd tower plate,the reflux ratio is 3,the plate number is 29,and the distillation ratio is 0.68.Under above conditions,the mass fraction of BDO at the top of the tower can reach 95.87%.By comparing the simulation results with the experimental results,the feasibility of the process and the reliability of the simulation results are verified.
Progress in Preparation of Nano-silica
CAO Shu-chao, WU Lin, YI De-lian, QIN Xiao-rong, LIU Xia, SUN Shao-xue (Applied Chemistry Research Institute , Wuhan University of Science and Technology, Wuhan 430081 ,China)The qualities and applications in plastics, rubber, fiber and coating of nano-silica are summarized. The new technologies such as sol-gel method, reversed microemulsion method, rotating packed bed and the characters of these methods are introduced. At last, the paper forecasts the application fields of nano-silica.