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Taking 95% ethanol as the solvent, we extracted flavonoids, the active constituents from Morus alba L.by an ultrasonic method.Moreover, taking the extraction rate of flavonoids as an evaluation index, we optimized the extraction process by response surface methodologies on the basis of single-factor experiments, and studied the antioxidant activity of the flavonoids extract.The results show that the optimum extraction conditions can be determined as follows: the extraction temperature of 45.78 ℃,the extraction time of 38.33 min, and the solid-liquid ratio of 1∶25.52(g∶mL).Under above conditions, the extraction rate can reach 2.211%.In vitro antioxidant experiments indicate that the scavenging rate of 4.00 mL 0.05 mg·mL-1 DPPH free radicals is 62.21% by 2.00 mL 0.50 mg·mL-1 flavonoids extract from Morus alba L.,which is lower than that of the positive control VC with the same concentration(81.39%).The precision, stability, and repeatability of antioxidant experiments are good, and RSD values are 0.05%,0.15%,and 0.69%(n=5),respectively.The extraction method is simple, efficient, and reliable, which provides a theoretical basis for the subsequent development of Morus alba L..
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Basic Information:
China Classification Code:R284.2
Citation Information:
[1]ZHANG Guanghui,LIU Jingli,LONG Xu ,et al.Optimization in Extraction Process of Flavonoids from Morus alba L. by Response Surface Methodology and Its Antioxidant Activity in vitro[J].Chemistry ＆ Bioengineering,2023,40(03):44-48.
2023-03-13
2023-03-13