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Using 95% ethanol as an extraction solvent and extraction rate as an evaluation index,we optimized the extraction process of licoflavone from different producing areas by response surface methodologies.Moreover,using rutin as a reference substance,we determined the content of licoflavone from different producing areas by sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method.Furthermore,we compared in vitro antioxidant activity of licoflavone from different producing areas by scavenging DPPH free radicals.The results show that the optimum ethanol extraction conditions of licoflavone are determined as follows:the extraction temperature of 86.7 ℃,the extraction time of 4.0 h,and the liquid-solid ratio of 80.0∶1.0(mL∶g).Under above conditions,the contents of licoflavone in Gansu,Xinjiang,and Inner Mongolia are 18.22 mg·g-1,17.75 mg·g-1,and 16.82 mg·g-1,respectively,and the scavenging rates of DPPH free radicals are 63.82%,62.74%,and 51.41%,respectively.The order of the licoflavone content and the in vitro antioxidant activity of three producing areas is Gansu>Xinjiang>Inner Mongolia.This conclusion can be used as one of the references for origin identification and quality evaluation of licorice,and can provide help for the development and utilization of licorice.
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Basic Information:
China Classification Code:R285.5;R284
Citation Information:
[1]ZHANG Guanghui,ZHANG Shuan,JIN Ruyi ,et al.Extraction Process Optimization,Content Determination,and in vitro Antioxidant Activity of Licoflavone[J].Chemistry ＆ Bioengineering,2020,37(08):17-21.
Fund Information:
陕西省科技攻关计划项目(2016SF-102); 陕西省教委基金项目(19JK0235)
2019-10-29
2019
2020-05-27
2020
1
2020-08-14
2020-08-14