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Taking the contents of glycyrrhizic acid, total flavonoids, and tannin as evaluation indicators, we optimized the preparation process of licorice extract by response surface methodologies on the basis of single-factor experiments.Moreover, we analyzed the chemical compositions in the licorice extract by UPLC-MS/MS,and evaluated its in vitro antibacterial activity.The results indicate that the optimal preparation process of licorice extract is as follows: the aqueous ammonia volume fraction of 3.0%,the ethanol volume fraction of 75%,and the ultrasonic time of 25 min.Under above conditions, the yield of licorice extract is 38%,which contains 88.90 mg·g-1 of glycyrrhizic acid, 27.06 mg·g-1 of total flavonoids, and 8.66 mg·g-1 of tannin.A total of 28 chemical compositions are identified, which belong to flavonoids, triterpene saponins, and coumarins, etc.The licorice extract exhibits high antibacterial activity, with half-maximal inhibitory concentrations(IC50) of 0.55,0.68,and 0.35 mg·mL-1 against Staphylococcus aureus,Escherichia coli,and Bacillus subtilis,respectively.The multi-index optimized preparation process of licorice extract can maintain multiple active compositions at a relatively high level, effectively improving the utilization rate of licorice, and providing a certain theoretical and experimental basis for the quality control and further research of licorice extract.
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Basic Information:
China Classification Code:TQ464
Citation Information:
[1]WEI Delong,FENG Yanlin,LIU Qijun ,et al.Multi-Index Optimization in Preparation Process of Licorice Extract and Composition Analysis[J].Chemistry ＆ Bioengineering,2026,43(06):45-56.
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四川省科学技术厅国际科技创新合作项目(2020YFH0205)
2026-06-04
2026-06-04