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Based on the excited-state intramolecular proton transfer(ESIPT) mechanism, we designed and synthesized two novel fluorescent probes(EB-1 and EB-2) with benzoate derivatives as specific recognition groups.We characterized the structures of the probes by ~1HNMR,13CNMR,and HRMS.Moreover, we studied the optical properties, specificity, and sensitivity of the probes.Furthermore, we explored their potentials for biological applications through butyrylcholinesterase(BChE) inhibitor screening experiments and in vivo imaging experiments.The results show that the probes EB-1 and EB-2 trigger ESIPT fluorophore signal amplification through enzymatic hydrolysis reaction, achieving selective response to BChE activity.The detection limits of probes EB-1 and EB-2 for BChE are 129 and 34 ng·mL-1,respectively, and effectively distinguished between BChE and acetylcholinesterase.Probes EB-1 and EB-2 exhibit time-and dose-dependence.In biological applications, probe EB-1 successfully achieves real-time fluorescence tracing of BChE in HEK 293T cells, and the spatial distribution characteristics of BChE are observed in living zebrafish.As novel molecular tools, probes EB-1 and EB-2 hold great potential for clinical diagnosis and drug development of BChE-related diseases.
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Basic Information:
China Classification Code:O657.3;TP212.3;R313
Citation Information:
[1]WANG Weibo,SUN Jinyu,HU Yufei ,et al.Design and Biosensing Applications of ESIPT-Driven Fluorescent Probe for Butyrylcholinesterase Detection[J].Chemistry ＆ Bioengineering,2026,43(06):38-44.
Fund Information:
国家自然科学基金项目(32200949); 山西省自然科学基金项目(202403021212045); 山西省高等教育科技创新项目(2024L323)
2026-06-04
2026-06-04