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Through grafting spermine(Spm) onto the backbone of hydroxyethyl amylose(HEA),we prepared Spm-g-HEA polymer, and characterized the structure of the polymer via FTIR,~1HNMR,and TNBS method.Moreover, based on the electrostatic adsorption of Spm-g-HEA and plasmid pEGFP-N1,we prepared Spm-g-HEA/pDNA complexes with different mass ratios, and analyzed the particle size, Zeta potential, morphology, and stability of the complexes via dynamic light scattering, electrophoretic light scattering, transmission electron microscopy, and gel retardation assay.Furthermore, we evaluated the transfection efficiency and cytotoxicity of Spm-g-HEA/pDNA complexes on HEK-293T cells via inverted fluorescence microscopy, flow cytometry, and MTT assay.The results show that, when the molar ratio of Spm to HEA is 2∶1,Spm will be grafted onto HEA backbond with a grafting degree of 3.6(based on 100 glucose units).Spm-g-HEA can tightly adsorb pDNA and compress it into positively charged irregular spherical nanoparticles at 100-200 nm.When Spm-g-HEA is complexed with pDNA at a mass ratio of 16∶1,it will exhibit the highest transfection efficiency and non-cytotoxicity, indicating that Spm-g-HEA is expected to develop into a safe and efficient non-viral transgenic vector.
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Basic Information:
China Classification Code:O636.12;Q782
Citation Information:
[1]XIONG Xiaoqin,KANG Jiali,XIONG Qi ,et al.Preparation, Characterization, and in vitro Transfection of Spermine-Grafted Hydroxyethyl Amylose[J].Chemistry ＆ Bioengineering,2026,43(06):23-29.
Fund Information:
国家自然科学基金项目(81903115)
2024-10-14
2024
2024-11-06
2024-11-28
2024
1
2026-06-04
2026-06-04