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2013, 06, v.30 1-6
Application of Red Homologous Recombination in Gene Knockout of E.coli
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Published:   2013-06-25
Publication Date:   2013-06-25
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Abstract:

Gene knockout technique of Escherichia coli is developing rapidly.Emerging of various kinds of new technologies has simplified the genome modification of E.coli than before.Especially,the Red homologous recombination system becomes more and more mature in gene knockout of E.coli.In this article,a few different kinds of Red homologous recombination technologies were reviewed.The mechanism and application strategies of each method were analyzed with its characteristics and superiority highlighted.

References

[1]Russell C B,Thaler D S,Dahlquist F W.Chromosomal transfor-mation of Escherichia coli recD strains with linearized plasmids[J].Journal of Bacteriology,1989,171(5):2609-2013.

[2]Hamilton C M,Aldea M,Washburn B K,et al.New method for generating deletions and gene replacements in Escherichia coli[J].Journal of Bacteriology,1989,171(9):4617-4622.

[3]Link A J,Phillips D,Church G M.Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli:Application to open reading frame characterization[J].Jour-nal of Bacteriology,1997,179(20):6228-6237.

[4]Murphy K C.Use of bacteriophageλrecombination functions to promote gene replacement in Escherichia coli[J].Journal of Bac-teriology,1998,180(8):2063-2071.

[5]Muyrers J P P,Zhang Y M,Stewart A F.Techniques:Recombino-genic engineering-new options for cloning and manipulating DNA[J].Trends in Biochemical Sciences,2001,26(5):325-331.

[6]Poteete A R.What makes the bacteriophage lambda Red system useful for genetic engineering:Molecular mechanism and biological function[J].Fems Microbiology Letters,2001,201(1):9-14.

[7]Copeland N G,Jenkins N A,Court D L.Recombineering:A pow-erful new tool for mouse functional genomics[J].Nature Reviews Genetics,2001,2(10):769-779.

[8]Muniyappa K,Shaner S L,Tsang S S,et al.Mechanism of the con-certed action of recA protein and helix-destabilizing proteins in ho-mologous recombination[J].Proceedings of the National Academy of Sciences of the United States of America,1984,81(9):2757-2761.

[9]Takahashi N,Kobayashi I.Evidence for the double-strand break repair model of bacteriophage lambda recombination[J].Proceed-ings of the National Academy of Sciences of the United States of America,1990,87(7):2790-2794.

[10]Ellis H M,Yu D,DiTizio T,et al.High efficiency mutagenesis,repair,and engineering of chromosomal DNA using single-stran-ded oligonucleotides[J].Proceedings of the National Academy of Sciences of the United States of America,2001,98(12):6742-6746.

[11]Murphy K C.Lambda Gam protein inhibits the helicase and chi-stimulated recombination activities of Escherichia coli RecBCD enzyme[J].Journal of Bacteriology,1991,173(18):5808-5821.

[12]Warming S,Costantino N,Court D L,et al.Simple and highly ef-ficient BAC recombineering using galK selection[J].Nucleic Acids Research,2005,33(4):e36.

[13]Datsenko K A,Wanner B L.One-step inactivation of chromo-somal genes in Escherichia coli K-12using PCR products[J].Proceedings of the National Academy of Sciences of the United States of America,2000,97(12):6640-6645.

[14]Doublet B,Douard G,Targant H,et al.Antibiotic marker modifi-cations of lambda Red and FLP helper plasmids,pKD46and pCP20,for inactivation of chromosomal genes using PCR prod-ucts in multidrug-resistant strains[J].Journal of Microbiological Methods,2008,75(2):359-361.

[15]Broach J R,Hicks J B.Replication and recombination functions associated with the yeast plasmid,2μcircle[J].Cell,1980,21(2):501-508.

[16]Court D L,Sawitzke J A,Thomason L C.Genetic engineering u-sing homologous recombination[J].Annual Review of Genetics,2002,36:361-388.

[17]Orkin S.Molecular-Cloning——A Laboratory Manual,2nd Edi-tion-Sambrook J,Fritsch E F,Maniatis T[J].Nature,1990,343(6259):604-605.

[18]Herring C D,Glasner J D,Blattner F R.Gene replacement with-out selection:Regulated suppression of amber mutations in Esch-erichia coli[J].Gene,2003,311:153-163.

[19]Lee D J,Bingle L E H,Heurlier K,et al.Gene doctoring:A method for recombineering in laboratory and pathogenic Esche-richia coli strains[J].BMC Microbiology,2009,9:252.

[20]Hobman J L,Patel M D,Hidalgo-Arroyo G A,et al.Comparative genomic hybridization detects secondary chromosomal deletions in Escherichia coli K-12MG1655mutants and highlights insta-bility in the flhDCregion[J].Journal of Bacteriology,2007,189(24):8786-8792.

[21]Poteete A R,Fenton A C,Nadkarni A.Chromosomal duplica-tions and cointegrates generated by the bacteriophage lamdba Red system in Escherichia coli K-12[J].BMC Molecular Biolo-gy,2004,5:22.

[22]Murphy K C,Campellone K G.Lambda Red-mediated recombi-nogenic engineering of enterohemorrhagic and enteropathogenic E.coli[J].BMC Molecular Biology,2003,4:11.

[23]Blattner F R,Plunkett G3rd,Bloch C A,et al.The complete ge-nome sequence of Escherichia coli K-12[J].Science,1997,277(5331):1453-1462.

[24]Welch R A,Burland V,Plunkett G,et al.Extensive mosaic struc-ture revealed by the complete genome sequence of uropathogenic Escherichia coli[J].Proceedings of the National Academy of Sci-ences of the United States of America,2002,99(26):17020-17024.

[25]Jamsai D,Orford M,Nefedov M,et al.Targeted modification of ahuman beta-globin locus BAC clone using GET recombination and an I-Sce I counterselection cassette[J].Genomics,2003,82(1):68-77.

[26]Tischer B K,von Einem J,Kaufer B,et al.Two-step red-media-ted recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli[J].Biotechniques,2006,40(2):191-197.

[27]Yu B J,Kang K H,Lee J H,et al.Rapid and efficient construc-tion of markerless deletions in the Escherichia coli genome[J].Nucleic Acids Research,2008,36(14):e84.

[28]Choulika A,Perrin A,Dujon B,et al.Induction of homologous re-combination in mammalian chromosomes by using the I-Sce I system of Saccharomyces cerevisiae[J].Molecular and Cellular Biology,1995,15(4):1968-1973.

[29]Rong Y S,Titen S W,Xie H B,et al.Targeted mutagenesis by homologous recombination in D.melanogaster[J].Genes Devel-opment,2002,16(12):1568-1581.

[30]Schmidt-Puchta W,Orel N,Kyryk A,et al.Intrachromosomal homologous recombination in Arabidopsis thaliana[J].Methods in Molecular Biology,2004,262:25-34.

Basic Information:

China Classification Code:Q78

Citation Information:

[1]L Shen-cong,ZHAO Ying-ying,ZHONG Wei-hong(College of Biological and Environmental Engineering, Zhejiang University of Technology,Hangzhou 310032,China).Application of Red Homologous Recombination in Gene Knockout of E.coli[J].Chemistry & Bioengineering,2013,30(06):1-6.

Fund Information:

国家自然科学基金资助项目(21076195); 教育部博士点基金项目(2011331711004); 浙江省科技厅国际合作项目(2011C24007)

Published:  

2013-06-25

Publication Date:  

2013-06-25

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