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According to the characteristic absorption of complex formed by I~-3 and starch,the activity of α-amylase is determined by visible spectrophotometry,which is determined through the amount of starch hydrolyzed before and after adding α-amylase.From the experimental results,we find that the maximum absorbance of complex is at 580 nm,and the color is stability in Na_2HPO4-citric acid buffer with pH value of 6.5.When a certain amount of starch is added,changing the concentration of α-amylase from 0.001-0.008 mg·mL-1,there is a good linear relationship between the concentration(x) and the activity(y) of α-amylase,and the linear equation is y=375.75x+0.1208(R2=0.9939).This method can be used to determine the activity of α-amylase.
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Basic Information:
China Classification Code:O657.3;R341
Citation Information:
[1]ZHANG Xuejiao,TIAN Huan,LIU Chunye ,et al.Determination of the Activity of α-Amylase by Visible Spectrophotometry[J].Chemistry ＆ Bioengineering,2020,37(03):65-68.
Fund Information:
陕西省教育厅项目(16JK1652,18JK0665); 西安医学院校级项目(2017PT05,2019YU032)
2019-11-26
2019
2020-01-08
2020
1
2020-04-25
2020-04-25