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We used high salt and high glycerine hyperosmotic stress to regulate the multiple production of active substances such as glucosylglycerol(GG),protein, and polysaccharides in Arthrospira platensis.Moreover, we investigated biomass accumulation, glycerine comsumption, photosynthetic efficiency, and multiple production of active substances such as GG,protein, and polysaccharides of Arthrospira platensis under different NaCl concentrations and different glycerine concentrations.The results show that high glycerine stress is an effective strategy for regulating the production of GG in Arthrospira platensis.With the addition of 20 g·L-1 glycerine, the GG content in Arthrospira platensis increases by 5.5 times, reaching 9.1% of biomass, and the GG yield reaches 201.1 mg·L-1,which is higher than the GG content and yield of most natural cyanobacteria under high salt stress.High salt stress inhibits protein production in Arthrospira platensis,while high glycerine stress increases protein yield to 928.3 mg·L-1.Under hyperosmotic stress, the protein produced by Arthrospira platensis is of high quality(EAAI>1),and its essential amino acid(EAA) content is higher than the FAO/WHO standard.High glycerine stress is beneficial for the production of total aromatic amino acids(especially phenylalanine),while high salt stress is beneficial for the production of medicinal amino acids glutamic acid and aspartic acid.High glycerine stress promots the production of polysaccharides in Arthrospira platensis,with a polysaccharides content reaching up to 21.9% of biomass under 10 g·L-1 glycerine and a yield of 426.0 mg·L-1 under 20 g·L-1 glycerine.The study provides a theoretical basis and technical reference for regulating the mutiple production of active substances in Arthrospira platensis via hyperosmotic stress.
[1] KWEI C K,LEWIS D,KING K,et al.Molecular classification of commercial Spirulina strains and identification of their sulfolipid biosynthesis genes[J].Journal of Microbiology and Biotechnology,2011,21(4):359-365.
[2] 李羚，高云涛，戴云，等.螺旋藻及螺旋藻多糖体外清除活性氧及抗氧化作用研究[J].化学与生物工程，2007,24(3):55-57.LI L,GAO Y T,DAI Y,et al.Scavenging effects of Spirulina and polysaccharides Spirulina platensis on active oxygens and its antioxidation in vitro[J].Chemistry & Bioengineering,2007,24(3):55-57.
[3] BREMER E,KRÄMER R.Responses of microorganisms to osmotic stress[J].Annual Review of Microbiology,2019,73:313-334.
[4] GUPTA A,SINGH S.Characterization of NaCl-tolerant mutant strain of the cyanobacterium Spirulina platensis overproducing phycocyanin[J].The Natural Products Journal,2017,7(1):1-12.
[5] LUO Q,DUAN Y K,LU X F.Biological sources,metabolism,and production of glucosylglycerols,a group of natural glucosides of biotechnological interest[J].Biotechnology Advances,2022,59:107964.
[6] SCHAGEN S K,OVERHAGEN S,BILSTEIN A.New data confirm skin revitalizing and stress protection by Glycoin® natural[J].Euro Cosmetics,2017(1/2):24-27.
[7] SARSEKEYEVA F K,SADVAKASOVA A K,SANDYBAYEVA S K,et al.Microalgae- and cyanobacteria-derived phytostimulants for mitigation of salt stress and improved agriculture[J].Algal Research,2024,82:103686.
[8] NARAYAN M S,MANOJ G P,VATCHRAVELU K,et al.Utilization of glycerol as carbon source on the growth,pigment and lipid production in Spirulina platensis[J].International Journal of Food Sciences and Nutrition,2005,56(7):521-528.
[9] PETELENZ-KURDZIEL E,KUEHN C,NORDLANDER B,et al.Quantitative analysis of glycerol accumulation,glycolysis and growth under hyper osmotic stress[J].PLoS Computational Biology,2013,9(6):e1003084.
[10] MARKOU G,KOUGIA E,KEFALOGIANNI I,et al.Effect of glycerol concentration and light intensity on growth and biochemical composition of Arthrospira(Spirulina) platensis:a study in semi-continuous mode with non-aseptic conditions[J].Applied Sciences,2019,9(21):4703.
[11] MARKOU G,CHATZIPAVLIDIS I,GEORGAKAKIS D.Effects of phosphorus concentration and light intensity on the biomass composition of Arthrospira(Spirulina) platensis[J].World Journal of Microbiology and Biotechnology,2012,28(8):2661-2670.
[12] YAO C H,AI J N,CAO X P,et al.Salinity manipulation as an effective method for enhanced starch production in the marine microalga Tetraselmis subcordiformis[J].Bioresource Technology,2013,146:663-671.
[13] QI M,YAO C H,SUN B H,et al.Application of an in situ CO2-bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis[J].Biotechnology for Biofuels,2019,12:184.
[14] RAUSCH T.The estimation of micro-algal protein content and its meaning to the evaluation of algal biomass Ⅰ.comparison of methods for extracting protein[J].Hydrobiologia,1981,78:237-251.
[15] XIANG Q,WEI X L,YANG Z Z,et al.Acclimation to a broad range of nitrate strength on a euryhaline marine microalga Tetraselmis subcordiformis for photosynthetic nitrate removal and high-quality biomass production[J].Science of the Total Environment,2021,781:146687.
[16] ZHANG T,CHI Z M,SHENG J.A highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a potentially useful for single-cell protein production and its nutritive components[J].Marine Biotechnology,2009,11(2):280-286.
[17] HUANG J K,YAO T,YI S J,et al.Effects of salt concentration and trophic mode on glucosylglycerol and phycocyanin production by Arthrospira maxima[J].Algal Research,2025,88:104025.
[18] BRISSON D,VOHL M C,ST-PIERRE J,et al.Glycerol:a neglected variable in metabolic processes?[J].BioEssays,2001,23(6):534-542.
[19] MARTNEZ-GOMEZ K,FLORES N,CASTAEDA H M,et al.New insights into Escherichia coli metabolism:carbon scavenging,acetate metabolism and carbon recycling responses during growth on glycerol[J].Microbial Cell Factories,2012,11(1):46.
[20] LU C M,ZHANG J H.Role of light in the response of PSⅡ photochemistry to salt stress in the cyanobacterium Spirulina platensis[J].Journal of Experimental Botany,2000,51(346):911-917.
[21] SHARMA G,KUMAR M,ALI M I,et al.Effect of carbon content,salinity and pH on Spirulina platensis for phycocyanin,allophycocyanin and phycoerythrin accumulation[J].Microbial & Biochemical Technology,2014,6(4):202-206.
[22] MARIN K,KANESAKI Y,LOS D A,et al.Gene expression profiling reflects physiological processes in salt acclimation of Synechocystis sp.strain PCC6803[J].Plant Physiology,2004,136(2):3290-3300.
[23] WARR S R,REED R H,CHUDEK J A,et al.Osmotic adjustment in Spirulina platensis[J].Planta,1985,163(3):424-429.
[24] FA Y,LIANG W H,CUI H,et al.Capillary ion chromatography-mass spectrometry for simultaneous determination of glucosylglycerol and sucrose in intracellular extracts of cyanobacteria[J].Journal of Chromatography B,2015,1001:169-173.
[25] ERDMANN N,FULDA S,HAGEMANN M.Glucosylglycerol accumulation during salt acclimation of two unicellular cyanobacteria[J].Journal of General Microbiology,1992,138(2):363-368.
[26] CUI J Y,SUN T,CHEN L,et al.Salt-tolerant Synechococcus elongatus UTEX2973 obtained via engineering of heterologous synthesis of compatible solute glucosylglycerol[J].Frontiers in Microbiology,2021,12:650217.
[27] 马培珍，谈晓明，吕雪峰，等.ggpS基因过表达对集胞藻PCC6803甘油葡萄糖苷和甘油合成的影响[J].生物工程学报，2016,32(3):347-354.MA P Z,TAN X M,LYU X F,et al.Effects of ggpS over-expression on glycosylglycerol and glycerol biosynthesis of Synechocystis sp.PCC6803[J].Chinese Journal of Biotechnology,2016,32(3):347-354.
[28] 马培珍.基因工程蓝细菌产甘油葡萄糖苷潜力研究[D].青岛：青岛农业大学，2016.MA P Z.Exploring the glucosylglycerol production capacity of genetically engineered cyanobacteria[D].Qingdao:Qingdao Agricultural University,2016.
[29] LUPATINI A L,COLLA L M,CANAN C,et al.Potential application of microalga Spirulina platensis as a protein source[J].Journal of the Science of Food and Agriculture,2017,97(3):724-732.
[30] MARKOU G,KOUGIA E,ARAPOGLOU D,et al.Production of Arthrospira platensis:effects on growth and biochemical composition of long-term acclimatization at different salinities[J].Bioengineering,2023,10(2):233.
[31] GABBAY-AZARIA R,SCHONFELD M,TEL-OR S,et al.Respiratory activity in the marine cyanobacterium Spirulina subsalsa and its role in salt tolerance[J].Archives of Microbiology,1992,157:183-190.
[32] YU C L,HU Y,ZHANG Y Q,et al.Concurrent enhancement of biomass production and phycocyanin content in salt-stressed Arthrospira platensis:a glycine betaine-supplementation approach[J].Chemosphere,2024,353:141387.
[33] SHIH I L,VAN Y T.The production of poly-(γ-glutamic acid) from microorganisms and its various applications[J].Bioresource Technology,2001,79(3):207-225.
[34] MORALES-SANCHEZ D,MARTINEZ-RODRIGUEZ O A,KYNDT J,et al.Heterotrophic growth of microalgae:metabolic aspects[J].World Journal of Microbiology & Biotechnology,2015,31(1):1-9.
[35] NAOI M,WU Y Q,MARUYAMA W,et al.Phytochemicals modulate biosynthesis and function of serotonin,dopamine,and norepinephrine for treatment of monoamine neurotransmission-related psychiatric diseases[J].International Journal of Molecular Sciences,2025,26(7):2916.
[36] HAYAMIZU K.Amino Acids and Energy Metabolism:An Overview[M]//BAGCHI D.Sustained Energy for Enhanced Human Functions and Activity.Academic Press,2017:339-349.
[37] 尹卫，梁健，王乐，等.QJ系列羊肚菌品种氨基酸特征评价[J].食品研究与开发，2024,45(4):49-57.YIN W,LIANG J,WANG L,et al.Evaluation of amino acid characteristics of QJ series of Morchella cultivars[J].Food Research and Development,2024,45(4):49-57.
[38] 张绪清.肝性脑病的预防与治疗进展[J].实用肝脏病杂志，2014,17(2):121-124.ZHANG X Q.Advances in prevention and treatment of hepatic encephalopathy[J].Journal of Practical Hepatology,2014,17 (2):121-124.
[39] BLAQUIERE C,BERTHON G.Speciation studies in relation to magnesium bioavailability.formation of Mg(Ⅱ) complexes with glutamate,aspartate,glycinate,lactate,pyroglutamate,pyridoxine and citrate,and appraisal of their potential significance towards magnesium gastrointestinal absorption[J].Inorganica Chimica Acta,1987,135(3):179-189.
[40] 谢成林，申雨韩，廖龙人，等.利用缺磷胁迫调控螺旋藻高效固碳生产功能性葡聚糖[J].食品与发酵工业，2025,51(16):115-125.XIE C L,SHEN Y H,LIAO L R,et al.Regulation of Arthrospira platensis for efficient carbon fixation and functional glucan production by phosphorus starvation[J].Food and Fermentation Industry,2025,51(16):115-125.
[41] LI J,ZHANG Y Q,YANG S,et al.Isolation,purification,characterization,and immunomodulatory activity analysis of α-glucans from Spirulina platensis[J].ACS Omega,2021,6(33):21384-21394.
[42] WHITE J J.Manipulation of storage polysaccharides in microorganisms[D].Edinburgh:University of Edinburgh,2016.
Basic Information:
China Classification Code:TQ920.1;Q949.9
Citation Information:
[1]CHEN Yuke,SHEN Yuhan,LIU Yufei ,et al.Regulation of Arthrospira platensis for Multiple Production of Active Substances via Hyperosmotic Stress[J].Chemistry ＆ Bioengineering,2026,43(05):39-48.
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四川省应用基础研究项目(2020YJ0069)
2025-05-13
2025
2025-06-24
2025
1
2026-01-09
2026-01-09
2026-01-09